Ghosh S, Poddar M K
Department of Biochemistry, University of Calcutta, India.
Methods Find Exp Clin Pharmacol. 1995 Apr;17(3):175-84.
The measurement of steady state level and accumulation rate of GABA and the activities of GAD and GABA-T in brain regions of adult male albino rats treated with diazepam (diazepam, 5 mg/kg/day, i.p.) and/or exposed (2 h/day) to higher environmental temperatures (higher environmental temperature, 40 degrees C) under long-term conditions (7-30 consecutive days) shows that long-term treatment with diazepam (5 mg/kg/day, i.p., for 15-30 consecutive days) reduced the GABAergic activity in the hypothalamus, corpus striatum (CS) and cerebellum (CM) without any alteration in the cerebrocortical region (CC). Multiple exposures to higher environmental temperatures for 7 consecutive days, on the other hand, enhanced GABAergic activity in hypothalamus, corpus striatum and cerebrocortical region of the rat. This higher environmental temperature-induced increase in GABAergic activity was further enhanced in the hypothalamus, but it was attenuated in the corpus striatum and became normalized in the cerebrocortical region upon the prolongation of exposure (15-30 consecutive days) to higher environmental temperature. The long-term diazepam-induced inhibition in GABAergic activity in hypothalamus and corpus striatum (but not in the cerebellum) disappeared and was finally activated following exposures (2 h/day) to higher environmental temperatures (40 degrees C) along with diazepam-treatment (5 mg/kg day, i.p.) for 7, 15 and 30 consecutive days. In the cerebellum, multiple exposures to higher environmental temperature did not significantly alter the long-term diazepam-induced inhibition of GABAergic activity or GABAergic activity in the normal rat.(ABSTRACT TRUNCATED AT 250 WORDS)
在长期条件下（连续7 - 30天），对成年雄性白化大鼠进行以下处理：腹腔注射地西泮（地西泮，5毫克/千克/天）和/或每天暴露于较高环境温度（较高环境温度，40摄氏度，每天2小时），然后测量大脑区域中γ-氨基丁酸（GABA）的稳态水平和积累速率以及谷氨酸脱羧酶（GAD）和GABA转氨酶（GABA-T）的活性。结果表明，长期注射地西泮（5毫克/千克/天，腹腔注射，连续15 - 30天）会降低下丘脑、纹状体（CS）和小脑（CM）中的GABA能活性，而大脑皮质区域（CC）则无任何变化。另一方面，连续7天多次暴露于较高环境温度会增强大鼠下丘脑、纹状体和大脑皮质区域的GABA能活性。这种较高环境温度诱导的GABA能活性增加在下丘脑中进一步增强，但在纹状体中减弱，并且在延长暴露于较高环境温度（连续15 - 30天）后，大脑皮质区域的GABA能活性恢复正常。长期地西泮诱导的下丘脑和纹状体（但不包括小脑）GABA能活性抑制在连续7天、15天和30天每天暴露于较高环境温度（40摄氏度）并同时注射地西泮（5毫克/千克/天，腹腔注射）后消失，并最终被激活。在小脑中，多次暴露于较高环境温度并未显著改变长期地西泮诱导的GABA能活性抑制或正常大鼠中的GABA能活性。（摘要截短于250字）
