Garrow Andrew G, Agnew Alison, Westhead David R
School of Biochemistry and Microbiology, University of Leeds, Leeds LS2 9JT, UK.
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W188-92. doi: 10.1093/nar/gki384.
TMB-Hunt is a program that uses a modified k-nearest neighbour (k-NN) algorithm to classify protein sequences as transmembrane beta-barrel (TMB) or non-TMB on the basis of whole sequence amino acid composition. By including differentially weighted amino acids, evolutionary information and by calibrating the scoring, a discrimination accuracy of 92.5% was achieved, as tested using a rigorous cross-validation procedure. The TMB-Hunt web server, available at www.bioinformatics.leeds.ac.uk/betaBarrel, allows screening of up to 10,000 sequences in a single query and provides results and key statistics in a simple colour coded format.
TMB-Hunt是一个程序，它使用改进的k近邻（k-NN）算法，根据整个序列的氨基酸组成将蛋白质序列分类为跨膜β桶（TMB）或非TMB。通过纳入差异加权氨基酸、进化信息并校准评分，使用严格的交叉验证程序进行测试时，实现了92.5%的判别准确率。TMB-Hunt网络服务器可在www.bioinformatics.leeds.ac.uk/betaBarrel上访问，允许在单个查询中筛选多达10000个序列，并以简单的颜色编码格式提供结果和关键统计信息。
