Suzuki K, Tomasi T B
J Exp Med. 1979 Oct 1;150(4):898-908. doi: 10.1084/jem.150.4.898.
The primary IgM antibody response to sheep erythrocytes in vivo as well as in vitro is markedly decreased in the spleen cells of pregnant mice, compared to age-matched female controls. Decreased antibody synthesis appears to be mediated by nonspecific suppressor cells, because the addition of pregnant spleen cells to the normal spleen cell cultures causes a significant suppression of plaque-forming-cell responses of the normal spleen cells. Suppressor cell activity was not observed in lymph nodes of pregnant mice. At least two populations of pregnant spleen cells were shown to exert a suppressor cell activity; one is T lymphocytes and the other a nylon-adherent cell present in the B-cell-enriched macrophage-depleted fraction. Pregnant spleen cells cultured in vitro were shown to secrete a soluble suppressive factor(s) into the supernatant medium.
与年龄匹配的雌性对照相比，怀孕小鼠脾细胞对绵羊红细胞的体内及体外主要IgM抗体反应显著降低。抗体合成减少似乎是由非特异性抑制细胞介导的，因为将怀孕小鼠的脾细胞添加到正常脾细胞培养物中会显著抑制正常脾细胞的空斑形成细胞反应。在怀孕小鼠的淋巴结中未观察到抑制细胞活性。已证明至少有两类怀孕小鼠的脾细胞具有抑制细胞活性；一类是T淋巴细胞，另一类是存在于富含B细胞、耗尽巨噬细胞的组分中的尼龙黏附细胞。体外培养的怀孕小鼠脾细胞被证明可向上清培养基中分泌一种可溶性抑制因子。
