使用分类群特异性定量PCR分析法评估土壤微生物群落结构。
Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays.
作者信息
Fierer Noah, Jackson Jason A, Vilgalys Rytas, Jackson Robert B
机构信息
Department of Biology, Duke University, Durham, North Carolina 27708, USA.
出版信息
Appl Environ Microbiol. 2005 Jul;71(7):4117-20. doi: 10.1128/AEM.71.7.4117-4120.2005.
Abstract
Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.
摘要
在此，我们描述了一种基于定量PCR的方法，用于估算土壤中细菌和真菌主要分类群的相对丰度。对引物的特异性进行了全面测试，并将该方法应用于三种不同的土壤。该技术提供了一种快速且可靠的微生物群落结构指标。
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