Rylander Lars, Nilsson-Ehle Peter, Hagmar Lars
Department of Occupational and Environmental Medicine, Institute of Laboratory Medicine, Lund University Hospital, SE-221 85 Lund, Sweden.
Chemosphere. 2006 Jan;62(3):333-6. doi: 10.1016/j.chemosphere.2005.04.107. Epub 2005 Jul 11.
In order to obtain biomarker values that reflect body burden of persistent organohalogen pollutants (POP), concentrations of lipophilic POP in serum or plasma are generally expressed on a lipid weight basis, and not on a fresh weight basis. There are two different approaches to determine the lipid content in serum and plasma. The gravimetric determination is more expensive, and longer experience is needed as compared with the preferable enzymatic determination. Clinical chemistry laboratories at most hospitals perform enzymatic determinations of cholesterol and triglycerides on a routine basis, whereas analysis of phospholipids is not part of these analyses. In the present study, therefore, we evaluated the association between the sum of measured triglyceride, cholesterol, and phospholipid levels in serum among 617 individuals with the sum of only triglyceride and cholesterol levels. There was a very strong linear association between the sums of serum concentrations of triglycerides and cholesterols and the total lipid concentrations. As much as 97.2% of the variation in total lipid concentrations was explained by the sum of the triglyceride and cholesterol concentrations, with the following regression: Total lipid=0.9+1.3 *(Cholesterol+Triglycerides). Almost identical relationships were recorded in subgroups with men, women and subjects of different age. Thus, total lipid concentrations in blood serum can be accurately estimated by analyzing only cholesterol and triglycerides in the matrix.
为了获得反映持久性有机卤素污染物（POP）体内负荷的生物标志物值，血清或血浆中亲脂性POP的浓度通常以脂质重量为基础表示，而不是以鲜重为基础。有两种不同的方法来测定血清和血浆中的脂质含量。重量法测定成本更高，与更可取的酶法测定相比，需要更长的经验。大多数医院的临床化学实验室常规进行胆固醇和甘油三酯的酶法测定，而磷脂分析不属于这些分析的一部分。因此，在本研究中，我们评估了617名个体血清中测得的甘油三酯、胆固醇和磷脂水平之和与仅甘油三酯和胆固醇水平之和之间的关联。甘油三酯和胆固醇的血清浓度之和与总脂质浓度之间存在非常强的线性关联。甘油三酯和胆固醇浓度之和解释了总脂质浓度高达97.2%的变异，回归方程如下：总脂质=0.9 + 1.3×（胆固醇 + 甘油三酯）。在男性、女性和不同年龄组的亚组中记录到几乎相同的关系。因此，通过仅分析基质中的胆固醇和甘油三酯，可以准确估计血清中的总脂质浓度。
