Yeh Su-Tuen, Lee Chiu-Sha, Chen Jiann-Chu
Department of Aquaculture, College of Life and Resource Sciences, National Taiwan Ocean University, Keelung 202, Taiwan, ROC.
Fish Shellfish Immunol. 2006 Mar;20(3):332-45. doi: 10.1016/j.fsi.2005.05.008.
The total haemocyte count (THC), phenoloxidase activity, and respiratory burst were examined when the white shrimp Litopenaeus vannamei (10.42+/-1.39 g) were immersed in seawater (34 per thousand) containing hot-water extract of brown alga Sargassum duplicatum at 100, 300 and 500 mg l(-1), or injected with hot-water extract of S. duplicatum at 2, 6, 10 and 20 microg g(-1). These parameters increased significantly when the shrimp were immersed in seawater containing hot-water extract at 300 and 500 mg l(-1) after 1 h, or when the shrimp were injected with hot-water extract at 10 and 20 microg g(-1) after 1 day. L. vannamei that were injected with hot-water extract at 6, 10 and 20 microg g(-1) had increased phagocytic activity and clearance efficiency to V. alginolyticus after 1-6 days. In another experiment, L. vannamei which had been immersed in seawater containing hot-water extract at 100, 300 and 500 mg l(-1), or injected with hot-water extract at 2, 6, 10 or 20 microg g(-1) were challenged with V. alginolyticus at 1 x 10(6), or 1.4 x 10(6) colony-forming units (cfu) shrimp(-1), and then placed in seawater. The survival of shrimp that received hot-water extract at either dose was significantly higher than that of control shrimp after 2 days, as well as at the termination of the experiment (6 days after the challenge). It is therefore concluded that L. vannamei that were immersed in hot-water extract of S. duplicatum at 300 mg l(-1), or the shrimp that were injected with hot-water extract at 10 microg g(-1) or less had increased immune ability as well as resistance to V. alginolyticus infection.
当凡纳滨对虾（10.42±1.39克）分别浸泡于含有100、300和500毫克/升的褐藻半叶马尾藻热水提取物的海水（盐度34‰）中，或分别注射2、6、10和20微克/克的半叶马尾藻热水提取物后，检测其血细胞总数（THC）、酚氧化酶活性和呼吸爆发。在1小时后，当对虾浸泡于含有300和500毫克/升热水提取物的海水中时，或在1天后当对虾注射10和20微克/克热水提取物时，这些参数显著增加。注射6、10和20微克/克热水提取物的凡纳滨对虾在1至6天后对溶藻弧菌的吞噬活性和清除效率有所提高。在另一项实验中，将分别浸泡于含有100、300和500毫克/升热水提取物的海水，或分别注射2、6、10或20微克/克热水提取物的凡纳滨对虾，用1×10⁶或1.4×10⁶菌落形成单位（cfu）/只的溶藻弧菌进行攻毒，然后置于海水中。在2天后以及实验结束时（攻毒后6天），接受任一剂量热水提取物的对虾存活率均显著高于对照对虾。因此得出结论，浸泡于300毫克/升半叶马尾藻热水提取物中的凡纳滨对虾，或注射10微克/克及以下热水提取物的对虾，其免疫能力以及对溶藻弧菌感染的抵抗力均有所增强。
