Oglesbee Michael J, Herdman Anne V, Passmore Gregory G, Hoffman William H
Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA.
Clin Biochem. 2005 Oct;38(10):900-4. doi: 10.1016/j.clinbiochem.2005.05.011.
Diabetic ketoacidosis (DKA) represents a metabolic stress whose treatment induces a systemic proinflammatory cytokine profile and accentuates life-threatening acute complications. The present study determined whether serum levels of the major inducible 70-kDa heat shock protein (Hsp72), a modulator of cytokine expression, were influenced by DKA and its treatment.
Serum levels of Hsp72 and glucose were measured in five adolescents with type 1 diabetes mellitus (T1DM) prior to, during and following correction of severe DKA. Samples from nine relatively euglycemic T1DM patients served as controls.
DKA pre-treatment samples showed significant elevation in Hsp72 (40.8 +/- 6.9 ng/ml) relative to euglycemic T1DM controls (33.6 +/- 3.2 ng/ml) (P < 0.05). Treatment resulted in a decline in Hsp72 to control levels within 24 h, with Hsp72 and glucose levels being tightly correlated (r = 0.9258).
Extracellular Hsp72 is increased by DKA, paralleling changes in serum glucose levels.
糖尿病酮症酸中毒（DKA）是一种代谢应激状态，其治疗会引发全身促炎细胞因子谱，并加重危及生命的急性并发症。本研究旨在确定主要的诱导型70 kDa热休克蛋白（Hsp72）（一种细胞因子表达调节剂）的血清水平是否受DKA及其治疗的影响。
在5名1型糖尿病（T1DM）青少年患者纠正重度DKA之前、期间及之后，测量其血清Hsp72和葡萄糖水平。来自9名血糖相对正常的T1DM患者的样本作为对照。
与血糖正常的T1DM对照（33.6±3.2 ng/ml）相比，DKA治疗前样本的Hsp72显著升高（40.8±6.9 ng/ml）（P<0.05）。治疗导致Hsp72在24小时内降至对照水平，且Hsp72与葡萄糖水平密切相关（r = 0.9258）。
DKA会使细胞外Hsp72增加，与血清葡萄糖水平的变化平行。
