M Dhanya, Kumar Vadivel Jayanth, T N Umamaheswari, Jayaraman Selvaraj
Oral Medicine and Radiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, IND.
Centre of Molecular Medicine and Diagnostics (COMManD) Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, IND.
Cureus. 2024 Aug 8;16(8):e66474. doi: 10.7759/cureus.66474. eCollection 2024 Aug.
Introduction Oral potentially malignant disorders (OPMD) are abnormally altered tissues that could potentially develop into oral cancer. From the literature, it is understandable that not all OPMDs develop into oral cancer. Hence, it is essential to identify the high-risk lesions that are more likely to develop into oral cancer. Lipid peroxidase (LPO) is a byproduct of phospholipid metabolism, and its levels are an oxidative stress marker that can probably help us predict the onset of cancer in OPMDS. This study aimed to assess the levels of LPO in OPMD, oral cancer, and normal patients. Materials and methods The sample size estimated was 15 per group. There were four groups in total. The estimation was done with the Abbkine LPO enzyme-linked immunosorption assay (ELISA) kit (Atlanta, Georgia, USA). An enzyme-substrate reaction was carried out, and the degree of the color change was measured using a microplate reader. The values were tabulated, and statistics were carried out using Statistical Package for Social Sciences (SPSS) version 26.0 (IBM Corp., Armonk, NY, USA). Both descriptive and inferential statistics were carried out. Results LPO levels (nmol/L) in each of the four groups were as follows: Group 1 (oral cancer): 171.86±78.86, Group 2 (controls): 71.66±28.36, Group 3: (oral leukoplakia): 127.50±103.53, and Group 4 (oral submucous fibrosis and oral lichen planus): 100.39±41.06. The results, when compared, were statistically significant (P< 0.05). Discussion From the above results, it is understandable that oral cancer patients experience increased oxidative stress compared to the OPMD group. The current study concluded that the obtained results showed differences in LPO levels, suggesting LPO could be used as a marker and screening tool to assess the rate and severity of cellular damage in patients with oral potentially malignant disorders.
引言 口腔潜在恶性疾病（OPMD）是可能发展为口腔癌的异常改变组织。从文献中可知，并非所有OPMD都会发展为口腔癌。因此，识别更有可能发展为口腔癌的高危病变至关重要。脂质过氧化物酶（LPO）是磷脂代谢的副产物，其水平是一种氧化应激标志物，可能有助于我们预测OPMD中癌症的发生。本研究旨在评估OPMD、口腔癌和正常患者中LPO的水平。
材料与方法 每组估计样本量为15例。总共分为四组。使用Abbkine LPO酶联免疫吸附测定（ELISA）试剂盒（美国佐治亚州亚特兰大）进行估计。进行酶-底物反应，并使用酶标仪测量颜色变化程度。将数值制成表格，并使用社会科学统计软件包（SPSS）26.0版（美国纽约州阿蒙克市IBM公司）进行统计。进行了描述性统计和推断性统计。
结果 四组中每组的LPO水平（nmol/L）如下：第1组（口腔癌）：171.86±78.86，第2组（对照组）：71.66±28.36，第3组（口腔白斑）：127.50±103.53，第4组（口腔黏膜下纤维化和口腔扁平苔藓）：100.39±41.06。比较结果具有统计学意义（P<0.05）。
讨论 从上述结果可知，与OPMD组相比，口腔癌患者经历了更高的氧化应激。当前研究得出结论，所获得的结果显示LPO水平存在差异，表明LPO可用作评估口腔潜在恶性疾病患者细胞损伤速率和严重程度的标志物和筛查工具。
