Bachand Marlene, Trent Amanda M, Bunker Bruce C, Bachand George D
Biomolecular Materials and Interfaces, Sandia National Laboratories, PO. Box 5800, MS 1413, Albuquerque, NM 87185-1413, USA.
J Nanosci Nanotechnol. 2005 May;5(5):718-22. doi: 10.1166/jnn.2005.112.
Recently, kinesin biomolecular motors and microtubules filaments (MTs) were used to transport metal and semiconductor nanoparticles with the long-term goal of exploiting this active transport system to dynamically assemble nanostructured materials. In some cases, however, the presence of nanoparticle cargo on MTs was shown to inhibit transport by interfering with kinesin-MT interactions. The primary objectives of this work were (1) to determine what factors affect the ability of kinesin and MTs to transport nanoparticle cargo, and (2) to establish a functional parameter space in which kinesin and MTs can support unimpeded transport of nanoparticles and materials. Of the factors evaluated, nanoparticle density on a given MT was the most significant factor affecting kinesin-based transport of nanoparticles. The density of particles was controlled by limiting the number of available linkage sites (i.e., biotinylated tubulin), and/or the relative concentration of nanoparticles in solution. Nanoparticle size was also a significant factor affecting transport, and attributed to the ability of particles < 40 nm in diameter to bind to the "underside" of the MT, and block kinesin transport. Overall, a generalized method of assembling and transporting a range of nanoparticle cargo using kinesin and MTs was established.
最近，驱动蛋白生物分子马达和微管丝（MTs）被用于运输金属和半导体纳米颗粒，其长期目标是利用这种主动运输系统动态组装纳米结构材料。然而，在某些情况下，MTs上纳米颗粒货物的存在被证明会通过干扰驱动蛋白与MT的相互作用来抑制运输。这项工作的主要目标是：（1）确定哪些因素会影响驱动蛋白和MT运输纳米颗粒货物的能力；（2）建立一个功能参数空间，在其中驱动蛋白和MT可以支持纳米颗粒和材料的无阻运输。在所评估的因素中，给定MT上的纳米颗粒密度是影响基于驱动蛋白的纳米颗粒运输的最重要因素。颗粒密度通过限制可用连接位点（即生物素化微管蛋白）的数量和/或溶液中纳米颗粒的相对浓度来控制。纳米颗粒大小也是影响运输的一个重要因素，这归因于直径小于40nm的颗粒能够结合到MT的“底面”并阻止驱动蛋白运输。总体而言，建立了一种使用驱动蛋白和MT组装和运输一系列纳米颗粒货物的通用方法。
