Taira Shu, Du Yong-Zhong, Hiratsuka Yuich, Uyeda Taro Q P, Yumoto Noboru, Kodaka Masato
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan.
Biotechnol Bioeng. 2008 Feb 15;99(3):734-9. doi: 10.1002/bit.21618.
We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 +/- 0.06 microm s(-1) at 25 degrees C) was comparable to that of control MTs. In addition, MTs conjugated with two types DNA sequences can achieve multiloading of Qds. To unload Qd molecular cargos from MTs, the DNA double helix connecting Qds to MTs were cleaved by an appropriate restriction enzyme. This biomolecular motors-based transport system should enable us to construct nanometer-scale devices such as nanobiosensor, nanofluidic system, or nanomachine.
我们开发了一种新方法，用于在驱动蛋白包被的表面上移动的微管（MTs）上加载和卸载分子货物。连接到21聚体DNA的量子点（Qds）（分子货物）可以通过DNA杂交选择性地加载到DNA共轭的MTs上。加载了Qd的MTs的平均速度（在25摄氏度下为0.43 +/- 0.06微米/秒）与对照MTs相当。此外，与两种类型DNA序列共轭的MTs可以实现Qds的多重加载。为了从MTs上卸载Qd分子货物，将连接Qds与MTs的DNA双螺旋用适当的限制酶切割。这种基于生物分子马达的运输系统应该能使我们构建纳米级设备，如纳米生物传感器、纳米流体系统或纳米机器。
