Zancan M, Franceschini R, Mimmo C, Vianello M, Di Tonno F, Mazzariol C, Malossini G, Gion M
ABO Association c/o Regional Center for the Study of Biological Markers of Malignancy, General Regional Hospital, Venice, Italy.
Int J Biol Markers. 2005 Apr-Jun;20(2):134-6. doi: 10.1177/172460080502000209.
The aim of the present preliminary study was to investigate the presence of free DNA (FDNA) in urine as a possible marker for the diagnosis of bladder cancer. Naturally voided morning urine specimens were collected from 57 patients with suspected bladder cancer before cystoscopy. A standard urine test was performed; the specimens were then processed in order to obtain a quantitative evaluation of the presence of free DNA in the urine. Twenty-two patients were excluded from the study because they had leukocyturia and/or bacteriuria. Free DNA concentrations higher than 250 ng/mL were found in all 16 patients showing bladder cancer at cystoscopy and in seven (36.8%) of the 19 patients with negative cystoscopy. Urinary FDNA seems to have an excellent sensitivity: we observed no false negative cases and 36.8% false positive cases. By contrast, only 6.25% of the bladder cancer patients had positive urine cytology. Our results seem promising, although further studies and larger numbers are needed to define urinary free DNA as a reliable marker of bladder cancer.
本初步研究的目的是调查尿液中游离DNA（FDNA）作为膀胱癌诊断潜在标志物的存在情况。在膀胱镜检查前，从57例疑似膀胱癌患者中收集晨尿标本。进行了标准尿液检测；然后对标本进行处理，以定量评估尿液中游离DNA的存在情况。22例患者因白细胞尿和/或菌尿被排除在研究之外。在膀胱镜检查显示患有膀胱癌的所有16例患者以及膀胱镜检查阴性的19例患者中的7例（36.8%）中，发现游离DNA浓度高于250 ng/mL。尿FDNA似乎具有出色的敏感性：我们未观察到假阴性病例，假阳性病例为36.8%。相比之下，只有6.25%的膀胱癌患者尿细胞学检查呈阳性。尽管需要进一步研究和更大样本量来将尿游离DNA定义为膀胱癌的可靠标志物，但我们的结果似乎很有前景。
