Park Yoon-Dong, Lee Hyang Burm, Yi Hana, Kim Youngjun, Bae Kyung Sook, Choi Jae-Eul, Jung Hack Sung, Chun Jongsik
School of Biological Sciences, Seoul National University, Kwanak-gu, Seoul 151-742, Republic of Korea.
Division of Biotechnology, The Catholic University of Korea, Puchon 420-743, Republic of Korea.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1721-1724. doi: 10.1099/ijs.0.63592-0.
A Gram-negative, aerobic bacterium, designated CG20106(T), was isolated from the surface tissues of rusty root lesions of Korean ginseng. Phylogenetic analysis of the 16S rRNA gene sequence revealed that this isolate represents a hitherto unknown subline within the genus Pseudomonas. Strain CG20106(T) was catalase- and oxidase-positive, motile and rod-shaped. The overall phenotypic features of the ginseng isolate were similar to those of Pseudomonas cedrina, Pseudomonas migulae and Pseudomonas azotoformans. However, several physiological and chemotaxonomic properties can be weighted to distinguish the isolate from these organisms. The major fatty acids were C(16:1)omega7c and/or iso-C(15:0) 2-OH (summed feature 3, 36.4+/-0.4%), C(16:0) (27.5+/-0.7%) and C(18:1)omega7c (19.4+/-0.2%). The DNA G+C content was 61.4 mol%. On the basis of the polyphasic results revealed in this study, the name Pseudomonas panacis sp. nov. is proposed for strain CG20106(T). The type strain is CG20106(T) (=IMSNU 14100(T)=CIP 108524(T)=KCTC 12330(T)).
从高丽参锈根病斑的表面组织中分离出一株革兰氏阴性需氧菌，命名为CG20106(T)。对其16S rRNA基因序列进行系统发育分析表明，该分离株代表假单胞菌属内一个迄今未知的亚系。菌株CG20106(T)过氧化氢酶和氧化酶呈阳性，具运动性，杆状。人参分离株的总体表型特征与雪松假单胞菌、米古拉假单胞菌和氮单胞菌假单胞菌相似。然而，可以根据一些生理和化学分类特性将该分离株与这些菌区分开来。主要脂肪酸为C(16:1)ω7c和/或异-C(15:0) 2-OH（特征性总和3，36.4±0.4%）、C(16:0)（27.5±0.7%）和C(18:1)ω7c（19.4±0.2%）。DNA G+C含量为61.4 mol%。基于本研究揭示的多相结果，提议将菌株CG20106(T)命名为人参假单胞菌（Pseudomonas panacis）新种。模式菌株为CG20106(T)（=IMSNU 14100(T)=CIP 108524(T)=KCTC 12330(T)）。
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