Bull Rowena A, Hansman Grant S, Clancy Leighton E, Tanaka Mark M, Rawlinson William D, White Peter A
School of Biotechnology and Biomolecular Sciences, Faculty of Science, University of New South Wales, Sydney, New South Wales, Australia.
Emerg Infect Dis. 2005 Jul;11(7):1079-85. doi: 10.3201/eid1107.041273.
Norovirus (NoV) genogroups I and II (GI and GII) are now recognized as the predominant worldwide cause of outbreaks of acute gastroenteritis in humans. Three recombinant NoV GII isolates were identified and characterized, 2 of which are unrelated to any previously published recombinant NoV. Using data from the current study, published sequences, database searches, and molecular techniques, we identified 23 recombinant NoV GII and 1 recombinant NoV GI isolates. Analysis of the genetic relationships among the recombinant NoV GII isolates identified 9 independent recombinant sequences; the other 14 strains were close relatives. Two of the 9 independent recombinant NoV were closely related to other recombinants only in the polymerase region, and in a similar fashion 1 recombinant NoV was closely related to another only in the capsid region. Breakpoint analysis of recombinant NoV showed that recombination occurred in the open reading frame (ORF)1/ORF2 overlap. We provide evidence to support the theory of the role of subgenomic RNA promoters as recombination hotspots and describe a simple mechanism of how recombination might occur in NoV.
诺如病毒（NoV）基因群I和II（GI和GII）现已被公认为全球人类急性胃肠炎暴发的主要病因。鉴定并表征了三株重组NoV GII分离株，其中两株与之前发表的任何重组NoV均无关联。利用本研究的数据、已发表序列、数据库搜索和分子技术，我们鉴定出23株重组NoV GII和1株重组NoV GI分离株。对重组NoV GII分离株之间的遗传关系分析确定了9个独立的重组序列；其他14株为近亲。9株独立的重组NoV中有两株仅在聚合酶区域与其他重组体密切相关，同样，1株重组NoV仅在衣壳区域与另一株密切相关。重组NoV的断点分析表明，重组发生在开放阅读框（ORF）1/ORF2重叠区。我们提供证据支持亚基因组RNA启动子作为重组热点的作用理论，并描述了NoV中重组可能发生的简单机制。
