Brain Repair Group, School of Biosciences, Cardiff University, Life Sciences Building, Museum Avenue, Cardiff, Wales, UK, CF10 3AX,
Transl Stroke Res. 2011 Dec;2(4):651-61. doi: 10.1007/s12975-011-0102-4. Epub 2011 Aug 16.
The intraluminal filament procedure is the most common model of middle cerebral artery occlusion (MCAO). However, consequences of subtle variations in surgical technique on behavioral outcome measures have not been sufficiently explored, which is the aim of this study. Rats randomly received one of three types of transient MCAO (60 min) surgeries. The first transected the external carotid artery (ECA) for filament insertion. The other two inserted the filament in the carotid artery (CA), and after reperfusion, the CA was either ligated or blood flow restored. All animals, including shams and naïve controls, were monitored with behavioral tests for 90 days. Lesion size and NeuN + cells in the striatum were comparable among MCAO groups. However, rats with ECA transection were consistently lighter than rats with permanent CA ligation, which were lighter than rats with CA reperfusion. Furthermore, rats with ECA transection exhibited the poorest lick efficiency and the greatest impairments in sensorimotor tasks. This study is the first to systematically evaluate the role of ECA transection on functional and morbidity outcomes. Behavioral impairments attributable to the surgical procedure were observed. This confounds studies and is an important issue that needs to be considered when using the intraluminal filament model.
管内栓线法是大脑中动脉阻塞（MCAO）最常用的模型。然而，手术技术的细微差异对行为结果测量的影响尚未得到充分探讨，这是本研究的目的。大鼠随机接受三种类型的短暂 MCAO（60 分钟）手术之一。第一种方法切断颈外动脉（ECA）以插入栓线。另外两种方法将栓线插入颈内动脉（CA），再灌注后，CA 要么结扎，要么恢复血流。所有动物，包括假手术和未处理对照组，均在 90 天内进行行为测试监测。MCAO 组之间的损伤大小和纹状体中的 NeuN+细胞无差异。然而，ECA 切断组的大鼠始终比永久性 CA 结扎组的大鼠轻，而 CA 再灌注组的大鼠比 CA 结扎组的大鼠轻。此外，ECA 切断组的大鼠表现出最差的舔效率和感觉运动任务的最大障碍。这项研究首次系统评估了 ECA 切断对功能和发病率结果的作用。观察到与手术程序相关的行为障碍。这会干扰研究，在使用管内栓线模型时是一个需要考虑的重要问题。
