Sohn Young-Soo, Goodey Adrian, Anslyn Eric V, McDevitt John T, Shear Jason B, Neikirk Dean P
The University of Texas at Austin, Department of Electrical and Computer Engineering, Austin, TX 78712, USA.
Biosens Bioelectron. 2005 Aug 15;21(2):303-12. doi: 10.1016/j.bios.2004.08.050. Epub 2004 Dec 2.
The development of a micromachined fluidic structure for the introduction of liquid samples into a chip-based sensor array composed of individually addressable polymeric microbeads is presented. The micromachined structure consists of micromachined storage cavities combined with a covering glass layer that confines the microbeads and fluidic channels. In our sensor array transduction occurs via optical (colorimetric and fluorescence) changes to receptors and indicator molecules that are covalently attached to termination sites on the polymeric microbeads. Spectral data are acquired for each of the individual microbeads using a charged-coupled device (CCD) allowing for the near-real-time analysis of liquid sample. Hence the micromachined fluidic structure must allow for both optical access to the microbeads and fluid flow through the micromachined cavities that serve as the microreactors/analysis chambers. One of the key parts of the structure is a passive fluid introduction system driven only by capillary force. This simple means of fluid introduction realizes a compact device. The capillary flow on the inlet channel has been studied, and the responses of the microbeads (alizarin complexone) to a liquid sample have been characterized. The test results show that this system is useful in a micro-total-analysis-system (mu-TAS) and biomedical applications.
本文介绍了一种微机械加工的流体结构，用于将液体样品引入由可单独寻址的聚合物微珠组成的基于芯片的传感器阵列。该微机械加工结构由微机械加工的储存腔与覆盖玻璃层组成，覆盖玻璃层可限制微珠和流体通道。在我们的传感器阵列中，转导通过与聚合物微珠末端位点共价连接的受体和指示剂分子的光学（比色和荧光）变化来实现。使用电荷耦合器件（CCD）获取每个单独微珠的光谱数据，从而实现对液体样品的近实时分析。因此，微机械加工的流体结构必须既允许对微珠进行光学访问，又允许流体流过用作微反应器/分析室的微机械加工腔。该结构的关键部分之一是仅由毛细作用力驱动的被动流体引入系统。这种简单的流体引入方式实现了设备的紧凑化。对入口通道上的毛细流动进行了研究，并对微珠（茜素络合酮）对液体样品的响应进行了表征。测试结果表明，该系统在微全分析系统（μ-TAS）和生物医学应用中很有用。
