van Woensel P A, van Blaaderen A, Moorman R J, de Boer G F
Central Veterinary Institute-DLO, Virology Department, Lelystad, The Netherlands.
Leukemia. 1992;6 Suppl 3:135S-137S.
Using molecular biological techniques, a study was made of the tissue tropism of avian leukosis virus (ALV) early after infection. Two strains of chickens, one with and the other without endogenous viral genes, were infected with ALV of subgroup A immediately after hatching; specimens of nine tissues and blood samples were analyzed at various times thereafter. A polymerase chain reaction (PCR), specific to ALV subgroup A, was used to detect proviral DNA and viral RNA. In situ hybridization was used to confirm the presence of proviral DNA in tissue samples and to calibrate the PCR. The pattern of detection of proviral DNA and of ALV-RNA in the various tissues was similar for both chicken strains. At 2 weeks of age, ALV-RNA was demonstrated in all tissues tested: bursa of Fabricius, thymus, bone marrow, proventriculus, liver, spleen, kidney, muscle, gonads, and blood samples, and at 4 weeks of age all tissues contained proviral DNA. No tropism for a specific tissue was observed early after an ALV infection.
利用分子生物学技术，对感染后早期禽白血病病毒（ALV）的组织嗜性进行了研究。两种品系的鸡，一种带有内源性病毒基因，另一种没有，孵化后立即用A亚群ALV感染；此后在不同时间对九个组织的标本和血样进行分析。使用针对A亚群ALV的聚合酶链反应（PCR）检测前病毒DNA和病毒RNA。原位杂交用于确认组织样本中前病毒DNA的存在并校准PCR。两种品系鸡的各个组织中前病毒DNA和ALV-RNA的检测模式相似。在2周龄时，在所有检测的组织中均检测到ALV-RNA：法氏囊、胸腺、骨髓、腺胃、肝脏、脾脏、肾脏、肌肉、性腺和血样，在4周龄时所有组织均含有前病毒DNA。在ALV感染后早期未观察到对特定组织的嗜性。
