Spiers Angela, Padmanabhan Neal
Division of Cardiovascular and Medical Sciences, University of Glasgow, UK.
Methods Mol Med. 2005;108:91-104. doi: 10.1385/1-59259-850-1:091.
Wire myography is an in vitro technique that allows us to examine functional responses and vascular reactivity of isolated small resistance arteries. Vessels from various species, including transgenic models, and vascular beds can be examined in a variety of pathological disease states. Vessels are dissected, cleaned, and then mounted onto a four-channel myograph under isometric techniques. Each vessel is then normalized to determine maximum active tension development. This allows the standardization of initial experimental conditions, an important consideration when examining pharmacological differences between vessels.
线肌动描记法是一种体外技术，它使我们能够检测离体小阻力动脉的功能反应和血管反应性。包括转基因模型在内的各种物种的血管以及不同的血管床都可以在多种病理疾病状态下进行检测。血管被解剖、清理，然后在等长技术下安装到四通道肌动描记仪上。然后对每根血管进行标准化处理，以确定最大主动张力的发展情况。这使得初始实验条件得以标准化，这在检测血管之间的药理学差异时是一个重要的考虑因素。
