del Campo Lara, Ferrer Mercedes
Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Vascular Biology Program, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029, Madrid, Spain.
Department of Physiology, Faculty of Medicine, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, 28029, Madrid, Spain.
Methods Mol Biol. 2015;1339:255-76. doi: 10.1007/978-1-4939-2929-0_18.
Atherosclerosis is characterized by endothelial dysfunction and alterations in vascular reactivity, which can be investigated by wire myography. The method allows ex vivo monitoring of the transversal isometric tension developed by a vessel segment in response to different pathophysiological stimuli. Here we describe in detail how to use the wire myograph to evaluate endothelial function and vasoconstrictor or vasodilator properties of the vessel, as well as to identify and characterize different factors and molecular pathways that control vascular tone. We also describe how to use the wire myograph to analyze biomechanical and passive properties of vessels such as diameter and elasticity.
动脉粥样硬化的特征是内皮功能障碍和血管反应性改变，这可以通过线肌描记法进行研究。该方法能够对血管段在不同病理生理刺激下产生的横向等长张力进行离体监测。在此，我们详细描述如何使用线肌描记仪来评估血管的内皮功能、血管收缩或舒张特性，以及识别和表征控制血管张力的不同因素和分子途径。我们还描述了如何使用线肌描记仪来分析血管的生物力学和被动特性，如直径和弹性。
