Szybalski E H, Szybalski W
Gene. 1979 Nov;7(3-4):217-70. doi: 10.1016/0378-1119(79)90047-7.
Physical and genetic mapping of deletion mutations has been correlated with the available molecular sizes of the lambda gene products and the DNA base sequence to construct a comprehensive molecular map of the phage lambda genome. The physical length of the DNA making up the left arm from the cos site through gene J is not sufficient to account in a nonoverlapping manner for all the proteins of the sizes reported to be coded, especially in the Nu1--C region. In the right arm all the coding capacity has not been accounted for, and it appears to be oversaturated only in the gam-ral region. The positions of several IS and Tn elements, and of restriction endonuclease cleavage sites are specified.
缺失突变的物理图谱和遗传图谱已与λ基因产物的现有分子大小及DNA碱基序列相关联，以构建噬菌体λ基因组的综合分子图谱。从粘性末端位点（cos site）到基因J构成左臂的DNA物理长度，不足以以不重叠的方式解释所有据报道编码的蛋白质大小，特别是在Nu1-C区域。在右臂中，所有的编码能力尚未得到解释，并且似乎仅在gam-ral区域过度饱和。指定了几个插入序列（IS）和转座子（Tn）元件的位置以及限制性内切酶切割位点的位置。
