Carr Jean K, Nadai Yuka, Eyzaguirre Lindsay, Saad Magdi D, Khakimov Mumtaz M, Yakubov Shavkat K, Birx Deborah L, Graham R Ross, Wolfe Nathan D, Earhart Kenneth C, Sanchez Jose L
US Military HIV Research Program, Henry M. Jackson Foundation, Rockville, MD 20850, USA.
J Acquir Immune Defic Syndr. 2005 Aug 15;39(5):570-5.
This research describes the genetic diversity of HIV-1 in Uzbekistan.
During 2002 and 2003, blood from HIV-positive patients in Uzbekistan was collected, and part of the proviral pol gene and nearly full-length genomes were sequenced and analyzed.
Among 142 Uzbek strains, most clustered genetically with the subtype A strain common in the former Soviet Union. Most of these subtype A-infected drug-naive subjects (65.6%) had an accessory drug resistance mutation, A62V, in the reverse transcriptase gene. Thirteen of the strains (9.2%) clustered with CRF02_AG, an HIV strain common in West Africa. People infected with CRF02_AG were all residents of Tashkent and sampled in 2002. The CRF02_AG strains were monophyletic and probably descended from a single ancestor. Two strains were recombinant between CRF02_AG and subtype A, with each having a different subtype structure. The CRF02_AG and the subtype A elements of the recombinants were monophyletic with Uzbek CRF02_AG and subtype A. New full-length genomes of 12 Uzbek strains suggested that neither the subtype A and nor the CRF02_AG strains in this epidemic were mosaics with other subtypes or circulating recombinant forms.
A genetic analysis of Uzbek HIV strains demonstrated the predominance of subtype A in the epidemic. An outbreak of a West African strain of HIV-1, CRF02_AG, occurred in Tashkent, Uzbekistan in 2002, however. The cocirculation of the 2 strains has resulted in new recombinants that are apparently unique to Uzbekistan.
本研究描述了乌兹别克斯坦HIV-1的基因多样性。
在2002年至2003年期间，收集了乌兹别克斯坦HIV阳性患者的血液，并对部分前病毒pol基因和几乎全长的基因组进行了测序和分析。
在142株乌兹别克菌株中，大多数在基因上与前苏联常见的A亚型菌株聚类。这些未接受过抗逆转录病毒治疗的A亚型感染患者中，大多数（65.6%）在逆转录酶基因中存在辅助性耐药突变A62V。其中13株菌株（9.2%）与CRF02_AG聚类，CRF02_AG是一种在西非常见的HIV菌株。感染CRF02_AG的人均为塔什干居民，于2002年采样。CRF02_AG菌株是单系的，可能源自单一祖先。有两株菌株是CRF02_AG与A亚型之间的重组体，各有不同的亚型结构。重组体的CRF02_AG和A亚型元件与乌兹别克CRF02_AG和A亚型是单系的。12株乌兹别克菌株的新全长基因组表明，该流行中的A亚型和CRF02_AG菌株均不是与其他亚型的嵌合体或流行重组形式。
对乌兹别克HIV菌株的基因分析表明，该流行中A亚型占主导地位。然而，2002年在乌兹别克斯坦塔什干爆发了一株西非HIV-1菌株CRF02_AG。这两种菌株的共同流行产生了显然是乌兹别克斯坦特有的新重组体。
