Ribeiro Daniel Araki, Duarte Marco Antonio Hungaro, Matsumoto Mariza Akemi, Marques Mariangela Esther Alencar, Salvadori Daisy Maria Favero
Center for Genotoxins and Carcinogens Evaluation (TOXICAN), Department of Pathology, Botucatu Medical School, UNESP, SP Brazil.
J Endod. 2005 Aug;31(8):605-7. doi: 10.1097/01.don.0000153842.06657.e2.
Mineral trioxide aggregate (MTA) and Portland cement are being used in dentistry as root end-filling materials. However, biocompatibility data concerning genotoxicity and cytotoxicity are needed for complete risk assessment of these compounds. In the present study, genotoxic and cytotoxic effects of MTA and Portland cements were evaluated in vitro using the alkaline single cell gel (comet) assay and trypan blue exclusion test, respectively, on mouse lymphoma cells. The results demonstrated that the single cell gel (comet) assay failed to detect DNA damage after a treatment of cells by MTA and Portland cements for concentrations up to 1000 microg/ml. Similarly, results showed that none of the compounds tested were cytotoxic. Taken together, these results seem to indicate that MTA and Portland cements are not genotoxins and do not induce cellular death.
矿物三氧化物凝聚体（MTA）和波特兰水泥在牙科中用作根尖充填材料。然而，为了对这些化合物进行全面的风险评估，需要有关遗传毒性和细胞毒性的生物相容性数据。在本研究中，分别使用碱性单细胞凝胶（彗星）试验和台盼蓝排斥试验，在体外对小鼠淋巴瘤细胞评估了MTA和波特兰水泥的遗传毒性和细胞毒性作用。结果表明，对于浓度高达1000微克/毫升的MTA和波特兰水泥处理的细胞，单细胞凝胶（彗星）试验未能检测到DNA损伤。同样，结果表明所测试的化合物均无细胞毒性。综上所述，这些结果似乎表明MTA和波特兰水泥不是遗传毒素，也不会诱导细胞死亡。
