Endodontic Area, Center for Dental Research, São Leopoldo Mandic University, Campinas, Brazil.
Int Endod J. 2010 Oct;43(10):843-8. doi: 10.1111/j.1365-2591.2010.01747.x. Epub 2010 Aug 16.
To evaluate whether white mineral trioxide aggregate (MTA) or white Portland cement with 15% bismuth oxide were able to induce genetic damage and cellular death ex vivo.
Aliquots of 1 × 10(4) murine fibroblasts were incubated at 37 °C for 3 h with MTA (white) or white Portland cement with 15% bismuth oxide, at final concentrations ranging from 10 to 1000 μg mL(-1) individually. Data of three independent repeats from the comet assay and the trypan blue exclusion test were assessed by the one-way anova followed by Tukey's test.
Mineral trioxide aggregate or Portland cement containing bismuth oxide did not produce genotoxic effects with respect to the single-cell gel (comet) assay data for all concentrations evaluated. Furthermore, no cytotoxicity was observed for MTA or Portland cement.
White MTA or white Portland cement containing 15% bismuth oxide were not genotoxic and cytotoxic.
评估白色矿化三氧化物聚合体（MTA）或含 15%氧化铋的白色波特兰水泥是否能在体外诱导遗传损伤和细胞死亡。
将 1×10(4)个鼠成纤维细胞分别在 37°C 下与 MTA（白色）或含 15%氧化铋的白色波特兰水泥孵育 3 小时，终浓度范围为 10 至 1000 μg/mL。单细胞凝胶电泳（彗星试验）和台盼蓝排斥试验的三个独立重复的数据采用单因素方差分析和 Tukey 检验进行评估。
就评估的所有浓度而言，矿化三氧化物聚合体或含氧化铋的波特兰水泥均未产生致突变作用的单细胞凝胶（彗星）试验数据。此外，MTA 或波特兰水泥均无细胞毒性。
白色 MTA 或含 15%氧化铋的白色波特兰水泥无遗传毒性和细胞毒性。
