Ngai Heidi H Y, Sit Wai-Hung, Wan Jennifer M E
Division of Food and Nutritional Sciences, Department of Zoology The University of Hong Kong, Hong Kong SAR, P.R. China.
Am J Chin Med. 2005;33(3):491-500. doi: 10.1142/S0192415X05003089.
In this study, we evaluated the renal protective effects of a Chinese herbal preparation WH30+ in male Wistar rats with glycerol-induced acute renal failure and adenine-induced chronic renal failure. WH30+ is a Chinese herb preparation composed of Rheum Palmatum, Salvia Miltiorrhiza, Cordyceps Sinensis, Leonurus Sibiricus, Epihedium Macranthum, Radix Astragali, and Radix Codonopsis Pilosulae, which has been used to treat kidney deficiency in human. An acute renal failure and chronic renal failure rat model were introduced by glycerol injection (i.m.) and fed with adenine-excessive diet, respectively. WH30+ was administered to rats at the dose of 50 mg/kg/day from 10 days before the diseases were induced until the rats were sacrificed. A reduction in body weight (p < 0.01) was observed in rats with chronic renal failure, but there was no difference between treatment groups. However, the body weight of rats with acute renal failure without treatment was significantly lower than those treated with WH30+ (p < 0.05). Overall, serum creatinine and urea nitrogen were elevated significantly (p < 0.01) in renal failure rats compared to control. Treatment with WH30+ improved both serum creatinine and urea nitrogen slightly in both models. The WH30+-treated rats with acute renal failure had significantly (p < 0.05) greater creatinine clearance than those without treatment. The results of the study show that WH30+ is more effective in the prevention of acute renal failure than chronic renal failure.
在本研究中，我们评估了一种中药制剂WH30+对甘油诱导的急性肾衰竭和腺嘌呤诱导的慢性肾衰竭雄性Wistar大鼠的肾脏保护作用。WH30+是一种由大黄、丹参、冬虫夏草、益母草、大花麻黄、黄芪和党参组成的中药制剂，已用于治疗人类肾虚。分别通过甘油注射（肌肉注射）和给予高腺嘌呤饮食建立急性肾衰竭和慢性肾衰竭大鼠模型。从诱导疾病前10天至大鼠处死，以50mg/kg/天的剂量给大鼠施用WH30+。观察到慢性肾衰竭大鼠体重下降（p<0.01），但各治疗组之间无差异。然而，未治疗的急性肾衰竭大鼠体重显著低于用WH30+治疗的大鼠（p<0.05）。总体而言，与对照组相比，肾衰竭大鼠的血清肌酐和尿素氮显著升高（p<0.01）。在两种模型中，用WH30+治疗均使血清肌酐和尿素氮略有改善。接受WH30+治疗的急性肾衰竭大鼠的肌酐清除率显著高于未治疗的大鼠（p<0.05）。研究结果表明，WH30+在预防急性肾衰竭方面比慢性肾衰竭更有效。
