College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
J Pharm Pharmacol. 2013 Sep;65(9):1409-18. doi: 10.1111/jphp.12110. Epub 2013 Jul 17.
Chronic renal failure (CRF) is a progressive, life-threatening condition with limited treatment options. Cordyceps sinensis is a fungus that has nephroprotective effects, and Isaria felina (IF) is a fungus isolated from C. sinensis fruiting bodies. We evaluated IF efficacy using an adenine-induced CRF animal model.
Forty male Sprague-Dawley rats were divided into normal control (n = 8) and adenine groups (n = 32; 100 mg/kg for 30 days). The adenine group was subdivided into a model control group (n = 7), a positive control group (200 mg/kg Jinshuibao capsule (JSB; n = 8), and two IF groups (200 mg/kg, n = 8; 100 mg/kg, n = 8). After treatment for 30 days, animals were narcotized and abdominal aortic blood was analysed. Kidney functions were evaluated.
Higher serum creatinine, blood urea nitrogen and uric acid levels, and lower creatinine clearance was observed in the model control group compared with JSB and IF groups (P < 0.05). Red blood cell count, haemoglobin and haematocrit levels in the 200 mg/kg IF group were higher than in the model control group (P < 0.05). Transforming growth factor-β1 mRNA expression in the model control group was higher than the normal control and 200 mg/kg IF groups (P < 0.05). Epidermal growth factor mRNA in the model control group was lower than in the normal control and both IF-treated groups (P < 0.05). Structural renal damage was observed in all adenine-treated rats, but was less severe in the JSB and IF groups.
IF may reverse the damaged kidney functions-induced with adenine in rats.
慢性肾衰竭（CRF）是一种进行性的、危及生命的疾病，治疗选择有限。冬虫夏草具有肾保护作用，而蝉拟青霉（IF）是从冬虫夏草子实体中分离出来的一种真菌。我们使用腺嘌呤诱导的 CRF 动物模型来评估 IF 的疗效。
将 40 只雄性 Sprague-Dawley 大鼠分为正常对照组（n=8）和腺嘌呤组（n=32；每天 100mg/kg，共 30 天）。腺嘌呤组再分为模型对照组（n=7）、阳性对照组（200mg/kg 金水宝胶囊（JSB；n=8）和两个 IF 组（200mg/kg，n=8；100mg/kg，n=8）。治疗 30 天后，对动物进行麻醉，分析腹主动脉血。评估肾功能。
与 JSB 和 IF 组相比，模型对照组的血清肌酐、血尿素氮和尿酸水平升高，肌酐清除率降低（P<0.05）。200mg/kg IF 组的红细胞计数、血红蛋白和血细胞比容水平高于模型对照组（P<0.05）。模型对照组的转化生长因子-β1 mRNA 表达高于正常对照组和 200mg/kg IF 组（P<0.05）。模型对照组的表皮生长因子 mRNA 低于正常对照组和两个 IF 治疗组（P<0.05）。所有腺嘌呤处理的大鼠均出现结构肾损伤，但 JSB 和 IF 组的损伤程度较轻。
IF 可能逆转腺嘌呤诱导的大鼠肾脏功能受损。
