Kirkwood J K, Wells G A, Cunningham A A, Jackson S I, Scott A C, Dawson M, Wilesmith J W
Institute of Zoology, Zoological Society of London.
Vet Rec. 1992 Apr 25;130(17):365-7. doi: 10.1136/vr.130.17.365.
A 19-month-old greater kudu (Tragelaphus strepsiceros), whose dam had died 15 months earlier with spongiform encephalopathy, required euthanasia after developing severe ataxia and depression with an apparently sudden onset. No macroscopic abnormalities were detected on post mortem examination but a scrapie-like spongiform encephalomyelopathy was apparent on histopathological examination of brain and segments of spinal cord. Negative stain electron microscopy of proteinase K-treated detergent extracts of tissue from the brain stem revealed the presence of scrapie associated fibrils, and a 25 to 28 kDa band comparable with that identified as abnormal PrP (prion protein) from the brains of domestic cattle with spongiform encephalopathy was detected using rabbit antiserum raised against mouse PrP. The animal was born nine months after the statutory ban on the inclusion of ruminant-derived protein in ruminant feeds and, as no other possible sources of the disease were apparent, it appears likely that the infection was acquired from the dam.
一只19个月大的大林羚（大羚羊），其母亲在15个月前死于海绵状脑病，在突然出现严重共济失调和抑郁后需要实施安乐死。尸检未发现宏观异常，但在对脑和脊髓节段进行组织病理学检查时发现了类似羊瘙痒病的海绵状脑脊髓病。对脑干组织经蛋白酶K处理的去污剂提取物进行负染电子显微镜检查，发现存在羊瘙痒病相关纤维，并且使用针对小鼠朊蛋白产生的兔抗血清检测到一条25至28 kDa的条带，该条带与从患有海绵状脑病的家牛大脑中鉴定为异常朊蛋白（PrP）的条带相当。这只动物在法定禁止在反刍动物饲料中添加反刍动物源性蛋白质九个月后出生，并且由于没有其他明显的可能疾病来源，感染似乎很可能是从其母亲那里获得的。
