Barriga O O, al-Khalidi N W, Martin S, Wyman M
Department of Veterinary Preventive Medicine, Ohio State University, Columbus 43210.
Vet Immunol Immunopathol. 1992 Apr;32(1-2):37-46. doi: 10.1016/0165-2427(92)90067-z.
Three clinically normal beagles, 3 beagles with localized demodectic mange (LDM), and 3 beagles with generalized demodectic mange (GDM) were investigated simultaneously 1-3 and 4-6 weeks from the appearance of the clinical signs. Blood clinical examination and reactivity of peripheral lymphocytes to Con A and PHA were investigated in the first instance, and reactivity to Con A, PHA, and LPS in the second. Eight aliquots were used in each blastogenesis assay for each dog. All dogs were negative for rheumatoid factor. The results of blastogenesis showed that many observations were distributed non-normally, and that not all dogs in each group responded homogeneously. Comparison of blastogenesis results between dogs demands careful statistical analysis. Responses to mitogens were normal in all dogs at 1-3 weeks except for the LDM dogs that showed an increased response to PHA. Only the response to Con A was moderately inhibited in the LDM dogs at 4-6 weeks. All responses were severely depressed in the GDM dogs at 4-6 weeks. This means that immunosuppression follows rather than precedes the clinical manifestations of GDM, and implies that the phenomenon is induced by the parasite or the host's reaction to it.
在临床症状出现后的1至3周以及4至6周，同时对3只临床正常的比格犬、3只患有局部蠕形螨病（LDM）的比格犬和3只患有全身性蠕形螨病（GDM）的比格犬进行了研究。首先调查了血液临床检查以及外周淋巴细胞对刀豆蛋白A（Con A）和植物血凝素（PHA）的反应性，其次调查了对Con A、PHA和脂多糖（LPS）的反应性。在每次淋巴细胞增殖试验中，每只狗使用8份样本。所有狗的类风湿因子均为阴性。淋巴细胞增殖结果表明，许多观察值呈非正态分布，且每组并非所有狗的反应都一致。比较不同狗之间的淋巴细胞增殖结果需要进行仔细的统计分析。在1至3周时，除LDM组的狗对PHA反应增强外，所有狗对有丝分裂原的反应均正常。在4至6周时，LDM组的狗仅对Con A的反应受到中度抑制。在4至6周时，GDM组的所有狗的反应均严重受抑。这意味着免疫抑制是在GDM临床表现之后而非之前出现，并且表明这种现象是由寄生虫或宿主对其的反应所诱导的。
