Paulík S, Mojzisová J, Bajová V, Baranová D, Paulíková I
University of Veterinary Medicine, Kosice, Slovak Republic.
Vet Med (Praha). 1996 Aug;41(8):245-9.
The aim of the study was to determine the degree of Con A induced lymphocyte blastogenesis in dogs with localized demodicosis (LD) within 1-3 and 6-8 weeks from appearance of the clinical signs. Ethidium bromide fluorescence assay was used for evaluation. In observation 9 clinically normal dogs, 6 dogs with LD a 4 dogs with generalized demodicosis (GD) were used. The results showed a statistically significant depression (P < 0.01) of blastogenesis in the LD dogs in comparison with that in the healthy dogs (Fig. 1). Responses to Con A were normal in dogs with LD in 1-3 weeks. However, a significantly depressed response to Con A (P < 0.025-0.001) was demonstrated in the LD dogs in 6-8 weeks (average 6.7 weeks) and it was comparable with that in the GD dogs with the duration of clinical disease on average for 8.7 weeks (Fig. 2; Tab. II). Thus, immunosuppression is not a necessary condition for dogs to develop spontaneous clinical LD and immunosuppression develops with the clinical signs of disease.
本研究的目的是确定在出现临床症状后的1 - 3周和6 - 8周内，患有局部蠕形螨病（LD）的犬中刀豆球蛋白A诱导的淋巴细胞增殖程度。采用溴化乙锭荧光测定法进行评估。观察对象包括9只临床正常犬、6只患有LD的犬和4只患有全身性蠕形螨病（GD）的犬。结果显示，与健康犬相比，LD犬的淋巴细胞增殖有统计学意义的降低（P < 0.01）（图1）。患有LD的犬在1 - 3周时对刀豆球蛋白A的反应正常。然而，在6 - 8周（平均6.7周）的LD犬中，对刀豆球蛋白A的反应显著降低（P < 0.025 - 0.001），且与临床疾病持续时间平均为8.7周的GD犬相当（图2；表II）。因此，免疫抑制不是犬发生自发性临床LD的必要条件，免疫抑制随着疾病的临床症状而出现。
