Kugelberg Elisabeth, Norström Tobias, Petersen Thomas K, Duvold Tore, Andersson Dan I, Hughes Diarmaid
Department of Cell and Molecular Biology, Box 596, The Biomedical Center, Uppsala University, S-751 24 Uppsala, Sweden.
Antimicrob Agents Chemother. 2005 Aug;49(8):3435-41. doi: 10.1128/AAC.49.8.3435-3441.2005.
A new animal model for the purpose of studying superficial infections is presented. In this model an infection is established by disruption of the skin barrier by partial removal of the epidermal layer by tape stripping and subsequent application of the pathogens Staphylococcus aureus and Streptococcus pyogenes. The infection and the infection route are purely topical, in contrast to those used in previously described animal models in mice, such as the skin suture-wound model, where the infection is introduced into the deeper layers of the skin. Thus, the present model is considered more biologically relevant for the study of superficial skin infections in mice and humans. Established topical antibiotic treatments are shown to be effective. The procedures involved in the model are simple, a feature that increases throughput and reproducibility. This new model should be applicable to the evaluation of novel antimicrobial treatments of superficial infections caused by S. aureus and S. pyogenes.
本文提出了一种用于研究浅表感染的新动物模型。在该模型中，通过胶带剥离部分去除表皮层破坏皮肤屏障，随后接种病原体金黄色葡萄球菌和化脓性链球菌来建立感染。与先前描述的小鼠动物模型（如皮肤缝合伤口模型，感染被引入皮肤深层）不同，该模型中的感染和感染途径完全是局部性的。因此，本模型被认为在研究小鼠和人类浅表皮肤感染方面具有更高的生物学相关性。已证实既定的局部抗生素治疗是有效的。该模型所涉及的操作简单，这一特点提高了通量和可重复性。这种新模型应适用于评估由金黄色葡萄球菌和化脓性链球菌引起的浅表感染的新型抗菌治疗方法。
