Klimowicz M, Lukaszewicz E, Dubiel A
Department and Clinic of Reproduction, Ruminant Diseases and Animal Health Protection, Wrocław Agricultural University, Wrocław, Poland.
Br Poult Sci. 2005 Jun;46(3):361-5. doi: 10.1080/00071660500098210.
The aim was to estimate the optimal frequency of semen collection from pigeons in relation to ejaculate volume, sperm concentration, total spermatozoa in ejaculate and percentage of live morphologically normal cells. The study was carried out on 455 ejaculates collected from two groups of pigeons, each of 10 males (group I: meat-type breed; group II: fancy pigeon). The birds were selected and kept individually in cages under a natural photoperiod. A two-person technique was used for semen collection (lumbo-sacral and cloacal region massage). Semen was collected once, twice or three times per week. Colour, consistency and volume of ejaculates were evaluated macroscopically immediately after collection. Sperm concentration and total number of cells in the ejaculate were estimated after dilution with Ringer's solution. A live-dead stain technique (nigrosin-eosin) was used to determine the percentage of live and normal spermatozoa. Semen collected 3x/week was of high quality. The average volume of a single ejaculate was small (21 microl in group I and 19 microl in group II), but sperm concentration was high--1.58 x 10(9)/ml and 1.96 x 10(9)/ml, respectively. The mean number of spermatozoa per ejaculate was 30.48 x 10(6) in group I and 39.49 x 10(6) in group II. An increased percentage of live and normal spermatozoa in semen collected more frequently was also observed. Collecting pigeon semen 3x/week provides spermatozoa in larger amounts and of better quality than less frequent collections (1x/week or 2x/week) and is recommended for obtaining more insemination doses.
目的是评估鸽子采精的最佳频率与射精量、精子浓度、射精中的总精子数以及形态正常的活细胞百分比之间的关系。该研究对从两组鸽子采集的455份精液进行，每组10只雄性鸽子（第一组：肉用型品种；第二组：观赏鸽）。挑选出这些鸽子并在自然光照周期下单独饲养在笼子里。采用两人技术进行采精（腰骶部和泄殖腔区域按摩）。每周采精一次、两次或三次。采精后立即肉眼评估射精的颜色、稠度和体积。用林格氏液稀释后估计射精中的精子浓度和细胞总数。采用死活染色技术（黑色素-伊红）确定活精子和正常精子的百分比。每周采精3次的精液质量高。单次射精的平均体积较小（第一组为21微升，第二组为19微升），但精子浓度较高，分别为1.58×10⁹/毫升和1.96×10⁹/毫升。第一组每次射精的平均精子数为30.48×10⁶，第二组为39.49×10⁶。还观察到更频繁采集的精液中活精子和正常精子的百分比增加。与较低频率的采精（每周1次或每周2次）相比，每周采精3次能提供数量更多、质量更好的精子，建议用于获得更多的输精剂量。
