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噬菌体φX174的A蛋白和A*蛋白对单链DNA的切割

Cleavage of single-stranded DNA by the A and A* proteins of bacteriophage phi X174.

作者信息

Langeveld S A, van Mansfeld A D, de Winter J M, Weisbeek P J

出版信息

Nucleic Acids Res. 1979 Dec 20;7(8):2177-88. doi: 10.1093/nar/7.8.2177.

DOI:10.1093/nar/7.8.2177
PMID:160544
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342378/
Abstract

The purified A protein and A* protein of bacteriophage phi X174 have been tested for endonuclease activity on single stranded viral phi X174 DNA. The A protein (55.000 daltons) nicks single-stranded DNA in the same way and at the same place as it does superhelical RFI DNA, at the origin of DNA replication. The A* protein (37.000 daltons) can cleave the single-stranded viral DNA at many different sites. It has however a strong preference for the origin of replication. Both proteins generate 3'OH ends and blocked 5' termini at the nick site.

摘要

已经对噬菌体φX174的纯化A蛋白和A蛋白进行了针对单链病毒φX174 DNA的核酸内切酶活性测试。A蛋白（55,000道尔顿）以与切割超螺旋RFI DNA相同的方式且在相同位置，即在DNA复制起点处切割单链DNA。A蛋白（37,000道尔顿）可在许多不同位点切割单链病毒DNA。然而，它强烈偏好复制起点。两种蛋白在切口位点均产生3'OH末端和封闭的5'末端。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/aa48838cfc73/nar00461-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/9ae3386f9f84/nar00461-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/e2019834e73c/nar00461-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/08d835c31989/nar00461-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/bc1ff2a06567/nar00461-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/acf7543f2f9d/nar00461-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/aa48838cfc73/nar00461-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/9ae3386f9f84/nar00461-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/e2019834e73c/nar00461-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/08d835c31989/nar00461-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/bc1ff2a06567/nar00461-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/acf7543f2f9d/nar00461-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2425/342378/aa48838cfc73/nar00461-0113-a.jpg

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引用本文的文献

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Cleavage of single-stranded DNA by the varphiX174 A protein: The A-single-stranded DNA covalent linkage.phiX174 A 蛋白对单链 DNA 的切割：A-单链 DNA 共价连接。
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4285-9. doi: 10.1073/pnas.81.14.4285.
2
The A* protein of phi X174 is an inhibitor of DNA replication.噬菌体φX174的A*蛋白是DNA复制的抑制剂。
Nucleic Acids Res. 1981 Apr 24;9(8):1991-2002. doi: 10.1093/nar/9.8.1991.
3
Cleavage and circularization of single-stranded DNA: a novel enzymatic activity of phi X174 A* protein.

本文引用的文献

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Formation of the parental replicative form DNA of bacteriophage phi-X174 and initial events in its replication.噬菌体φ-X174亲本复制型DNA的形成及其复制的起始事件。
J Mol Biol. 1971 Nov 14;61(3):565-86. doi: 10.1016/0022-2836(71)90065-9.
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DNA replication: the rolling circle model.DNA复制：滚环模型
Cold Spring Harb Symp Quant Biol. 1968;33:473-84. doi: 10.1101/sqb.1968.033.01.055.
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Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.噬菌体φX174的基因A起始子的分离及其功能，一种高度特异性的DNA内切酶
单链DNA的切割与环化：噬菌体φX174 A*蛋白的一种新酶活性
Nucleic Acids Res. 1980 Nov 25;8(22):5305-15. doi: 10.1093/nar/8.22.5305.
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Cleavage of phi X174 single-stranded DNA by gene A protein and formation of a tight protein-DNA complex.基因A蛋白对φX174单链DNA的切割及紧密蛋白-DNA复合物的形成。
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Initiation and termination of the bacteriophage phi X174 rolling circle DNA replication in vivo: packaging of plasmid single-stranded DNA into bacteriophage phi X174 coats.噬菌体φX174滚环DNA复制在体内的起始与终止：质粒单链DNA包装进噬菌体φX174衣壳
Nucleic Acids Res. 1982 Nov 11;10(21):6849-63. doi: 10.1093/nar/10.21.6849.
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Mechanism of replication of bacteriophage phi X174 XX. Sensitivity of nascent DNA to single-strand-specific nucleases.噬菌体φX174的复制机制XX. 新生DNA对单链特异性核酸酶的敏感性
J Virol. 1982 Apr;42(1):12-9. doi: 10.1128/JVI.42.1.12-19.1982.
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Enzymatic properties of the bacteriophage phi X174 A protein on superhelical phi X174 DNA: a model for the termination of the rolling circle DNA replication.噬菌体φX174 A蛋白对超螺旋φX174 DNA的酶学特性：滚环DNA复制终止的模型
Nucleic Acids Res. 1981 May 11;9(9):2037-53. doi: 10.1093/nar/9.9.2037.
8
Conservation of the primosome in successive stages of phi X174 DNA replication.噬菌体φX174 DNA复制连续阶段中引发体的保守性
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1436-40. doi: 10.1073/pnas.78.3.1436.
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The nuclease specificity of the bacteriophage phi X174 A* protein.噬菌体φX174 A*蛋白的核酸酶特异性
Nucleic Acids Res. 1981 Feb 11;9(3):545-62. doi: 10.1093/nar/9.3.545.
10
Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.大鼠肝脏切口-封闭酶对单链DNA的断裂及DNA-酶复合物的形成
Nucleic Acids Res. 1980 Dec 20;8(24):6129-42. doi: 10.1093/nar/8.24.6129.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549-53. doi: 10.1073/pnas.71.4.1549.
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Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳测定小双链和单链DNA分子的链长
Biochemistry. 1975 Aug 26;14(17):3787-94. doi: 10.1021/bi00688a010.
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Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA.噬菌体φX174 DNA的基因A所编码蛋白质的分离与特性分析
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2669-73. doi: 10.1073/pnas.73.8.2669.
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Gene A product of phi X174 is required for site-specific endonucleolytic cleavage during single-stranded DNA synthesis in vivo.噬菌体φX174的基因A产物是体内单链DNA合成过程中位点特异性内切核酸酶切割所必需的。
J Virol. 1976 Aug;19(2):416-24. doi: 10.1128/JVI.19.2.416-424.1976.
7
Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.用于鉴定和表征质粒脱氧核糖核酸的简易琼脂糖凝胶电泳方法。
J Bacteriol. 1976 Sep;127(3):1529-37. doi: 10.1128/jb.127.3.1529-1537.1976.
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'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.来自噬菌体φX174和M13的“单链”DNA被某些限制性核酸内切酶切割。
Nature. 1975 Oct 2;257(5525):421-2. doi: 10.1038/257421a0.
9
Bacteriophage phiX174 DNA synthesis in a replication-deficient host: determination of the origin of phiX DNA replication.在复制缺陷型宿主中噬菌体φX174 DNA的合成：φX DNA复制起点的确定
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The nucleotide sequence of bacteriophage phiX174.噬菌体φX174的核苷酸序列。
J Mol Biol. 1978 Oct 25;125(2):225-46. doi: 10.1016/0022-2836(78)90346-7.