Williams R O, Plater-Zyberk C, Williams D G, Maini R N
Kennedy Institute of Rheumatology, London, UK.
Clin Exp Immunol. 1992 Jun;88(3):455-60. doi: 10.1111/j.1365-2249.1992.tb06471.x.
We describe the adoptive transfer of erosive arthritis to an immunodeficient host. Spleen cells from arthritic DBA/1 mice (H-2q), immunized 4-6 weeks previously with bovine type II collagen in adjuvant, were transferred intraperitoneally into SCID mice (H-2d). SCID recipient mice also received native or denatured type II collagen (100 micrograms intraperitoneally) at the time of cell transfer. Arthritis developed in five out of five mice approximately 2 weeks after injection of cells plus native collagen, whereas animals injected with cells plus denatured collagen did not show any clinical or histological evidence of arthritis. The minimum graft size required for successful transfer of arthritis was established at 10(7) DBA/1 spleen cells. Histological examination of the joints of arthritic SCID recipient mice revealed synovitis, fibrosis and erosion of cartilage and underlying bone. Mean circulating levels of anti-type II collagen IgG were found to be significantly higher in mice injected with native collagen than those injected with denatured collagen (40 micrograms/ml and less than 1 microgram/ml, respectively). The ability to transfer collagen-induced arthritis adoptively should facilitate the study of the cellular requirement and pathological mechanisms involved in the induction of this arthropathy.
我们描述了将侵蚀性关节炎过继转移至免疫缺陷宿主的过程。来自关节炎DBA/1小鼠（H-2q）的脾细胞，于4 - 6周前用佐剂中的牛II型胶原免疫，经腹腔注射至SCID小鼠（H-2d）体内。SCID受体小鼠在细胞转移时还接受了天然或变性的II型胶原（腹腔注射100微克）。在注射细胞加天然胶原后约2周，五只小鼠中有五只发生了关节炎，而注射细胞加变性胶原的动物未显示出任何关节炎的临床或组织学证据。成功转移关节炎所需的最小移植物大小确定为10(7)个DBA/1脾细胞。对患有关节炎的SCID受体小鼠关节进行组织学检查，发现有滑膜炎、纤维化以及软骨和下方骨骼的侵蚀。发现注射天然胶原的小鼠中抗II型胶原IgG的平均循环水平显著高于注射变性胶原的小鼠（分别为40微克/毫升和低于1微克/毫升）。过继转移胶原诱导性关节炎的能力应有助于研究诱导这种关节病所涉及的细胞需求和病理机制。
