Andert S E, Griesmacher A, Zuckermann A, Müller M M
Second Department of Surgery, University of Vienna, Austria.
Clin Exp Immunol. 1992 Jun;88(3):555-8. doi: 10.1111/j.1365-2249.1992.tb06486.x.
Human umbilical vein endothelial cells (HUVEC) were investigated for their ability to produce neopterin, a biochemical marker for an activated immune system. Interferon-gamma (IFN-gamma), IL-1 alpha, IL-2, IL-6, tumour necrosis factor-alpha, granulocyte/macrophage colony stimulating factor, phytohaemagglutinin and concanavalin A were used to stimulate HUVEC. While IFN-gamma induced neopterin release from HUVEC in a time- and dose-dependent manner, all the other cytokines used had no effect on neopterin production. High neopterin levels are found in patients with rejection episodes or infections. Our results suggest that not only monocytes and macrophages, which are known to synthesize neopterin, but also endothelial cells are responsible for these high serum neopterin levels.
研究了人脐静脉内皮细胞（HUVEC）产生新蝶呤的能力，新蝶呤是活化免疫系统的一种生化标志物。使用干扰素-γ（IFN-γ）、白细胞介素-1α、白细胞介素-2、白细胞介素-6、肿瘤坏死因子-α、粒细胞/巨噬细胞集落刺激因子、植物血凝素和刀豆球蛋白A刺激HUVEC。虽然IFN-γ以时间和剂量依赖性方式诱导HUVEC释放新蝶呤，但所使用的所有其他细胞因子对新蝶呤的产生均无影响。在发生排斥反应或感染的患者中发现新蝶呤水平较高。我们的结果表明，不仅已知能合成新蝶呤的单核细胞和巨噬细胞，而且内皮细胞也与这些高血清新蝶呤水平有关。
