Elbeik Tarek, Nassos Patricia, Kipnis Patricia, Haller Barbara, Ng Valerie L
Clinical Laboratories at San Francisco General Hospital, Department of Laboratory Medicine, Building NH, Room 2M35, 1001 Potrero Ave., San Francisco, CA 94110, USA.
J Clin Microbiol. 2005 Aug;43(8):3769-71. doi: 10.1128/JCM.43.8.3769-3771.2005.
Separation and storage of plasma within 2 h of phlebotomy is required for the VACUTAINER PPT Plasma Preparation Tube (PPT) versus 4 h for the predecessor VACUTAINER EDTA tube for human immunodeficiency virus type 1 (HIV-1) viral load (HIVL) testing by the VERSANT HIV-1 RNA 3.0 assay (branched DNA). The 2-h limit for PPT imposes time constraints for handling and transporting to the testing laboratory. This study compares HIVL reproducibility from matched blood in EDTA tubes and PPTs and between PPT pairs following processing within 4 h of phlebotomy, stability of plasma HIV-1 RNA at 24- and 72-h room temperature storage in the tube, and comparative labor and supply requirements. Blood from 159 patients was collected in paired tubes (EDTA/PPT or PPT/PPT): 86 paired EDTA tubes and PPTs were processed 4 h following phlebotomy and their HIVLs were compared, 42 paired PPT/PPT pairs were analyzed for intertube HIVL reproducibility, and 31 PPT/PPT pairs were analyzed for HIV-1 RNA stability by HIVL. Labor and supply requirements were compared between PPT and EDTA tubes. PPTs produce results equivalent to standard EDTA tube results when processed 4 h after phlebotomy. PPT intertube analyte results are reproducible. An average decrease of 13% and 37% in HIVL was observed in PPT plasma after 24 and 72 h of room temperature storage, respectively; thus, plasma can be stored at room temperature up to 24 h in the original tube. PPTs offer labor and supply savings over EDTA tubes.
对于使用VERSANT HIV-1 RNA 3.0检测法（分支DNA法）进行人类免疫缺陷病毒1型（HIV-1）病毒载量（HIVL）检测而言，VACUTAINER PPT血浆制备管（PPT）要求在采血后2小时内分离并储存血浆，而其前身VACUTAINER EDTA管则要求在4小时内完成。PPT的2小时限制给处理和运输至检测实验室带来了时间限制。本研究比较了在采血后4小时内处理的EDTA管和PPT中匹配血液的HIVL重复性、血浆HIV-1 RNA在管中于室温储存24小时和72小时后的稳定性，以及劳动和耗材需求的比较。收集了159名患者的血液于配对管中（EDTA/PPT或PPT/PPT）：86对EDTA管和PPT在采血后4小时进行处理，并比较其HIVL；42对PPT/PPT用于分析管间HIVL重复性；31对PPT/PPT用于通过HIVL分析HIV-1 RNA稳定性。比较了PPT和EDTA管的劳动和耗材需求。采血后4小时处理时，PPT产生的结果与标准EDTA管结果相当。PPT管间分析物结果具有可重复性。室温储存24小时和72小时后，PPT血浆中的HIVL平均分别下降了13%和37%；因此，血浆可在原管中于室温储存长达24小时。与EDTA管相比，PPT节省了劳动和耗材。
