Lopez R, Tapia A, Portoles A
"Jaime Ferrán" Institute of Microbiology, C.S.I.C., Madrid, Spain.
Mol Gen Genet. 1975;136(1):87-94. doi: 10.1007/BF00275451.
Using a new method to get stable steady state levels of competence in B. subtilis, we have obtained in our experimental conditions 0.1% competence as determined by the frequency of transfection with phage SPP1 DNA. The effect of growth rate (DT) and certain amino acids on the competence levels was also determined.
我们使用一种新方法来获得枯草芽孢杆菌中稳定的感受态稳态水平，在我们的实验条件下，通过用噬菌体SPP1 DNA转染的频率测定，获得了0.1%的感受态。我们还测定了生长速率（DT）和某些氨基酸对感受态水平的影响。
