Pan Luqing, Ren Jiayun, Liu Jing
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2005 Jul;141(3):248-56. doi: 10.1016/j.cca.2005.07.005.
Scallops (Chlamys ferrari) were cultured for 30 days in seawater containing benzo(k)fluoranthene (BkF) at 0.5, 1.0 and 10.0 microg/L. No effects were noted on 7-ethoxyresorufin O-deethylase (EROD) activity in digestive gland at low concentrations (0.5 and 1 microg/L) of BkF, but BkF increased the glutathione-S-transferase (GST) activity. At 10 microg/L BkF increased EROD activity significantly, and depressed GST activity. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in digestive gland increased significantly in 0.5 and 1 microg/L BkF. In 10 microg/L concentrations of BkF, the activity of three antioxidant enzymes increased first and reached a peak after a few days, before tapering off towards the end of the 30 day exposure. In high concentrations of BkF, activity of three antioxidant enzymes in gill showed an early peak (12 h), before dropping off. Lipid peroxidation (LPO) levels increased along with sampling times, and there were time- and concentration-effects between LPO levels and BkF. The responses of the gills and the digestive gland were not always parallel which can be explained by differences in the bioavailability of the toxicant. The performance of each biomarker is assessed in the context of the role and advantages of selecting a battery of biomarkers for detecting contamination problems. The use of C. ferrari as a sentinel species for biomonitoring potential toxic effects in situ is discussed as well as mechanisms of BKF toxicity and alexipharmic strategies of C. ferrari.
将扇贝（费氏海扇）置于含有浓度分别为0.5、1.0和10.0微克/升苯并(k)荧蒽（BkF）的海水中养殖30天。低浓度（0.5和1微克/升）的BkF对消化腺中的7-乙氧基异吩唑酮-O-脱乙基酶（EROD）活性未产生影响，但BkF会增加谷胱甘肽-S-转移酶（GST）的活性。在10微克/升时，BkF显著提高了EROD活性，并降低了GST活性。在0.5和1微克/升的BkF作用下，消化腺中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）显著增加。在10微克/升的BkF浓度下，三种抗氧化酶的活性先升高，在几天后达到峰值，然后在30天暴露期结束时逐渐下降。在高浓度的BkF作用下，鳃中三种抗氧化酶的活性出现早期峰值（12小时），随后下降。脂质过氧化（LPO）水平随采样时间增加，LPO水平与BkF之间存在时间和浓度效应。鳃和消化腺的反应并不总是平行的，这可以通过毒物生物利用度的差异来解释。在选择一系列生物标志物以检测污染问题的作用和优势的背景下，评估了每个生物标志物的性能。还讨论了将费氏海扇用作原位生物监测潜在毒性效应的指示物种，以及BKF的毒性机制和费氏海扇的解毒策略。
