通过在藻酸盐微球上应用多层纳米薄膜涂层来稳定封装活性酶。

Stable encapsulation of active enzyme by application of multilayer nanofilm coatings to alginate microspheres.

作者信息

Srivastava Rohit, Brown Jonathan Q, Zhu Huiguang, McShane Michael J

机构信息

Biomedical Engineering and Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, USA.

出版信息

Macromol Biosci. 2005 Aug 12;5(8):717-27. doi: 10.1002/mabi.200500061.

DOI:10.1002/mabi.200500061

PMID:16096991

Abstract

In an effort to improve the stability for long-term biosensor use, layer-by-layer self-assembly was explored as a potential technique to provide a diffusion barrier to encapsulated glucose oxidase inside alginate microspheres (<5 microm), fabricated using an emulsification technique. The total loss of encapsulated enzyme was reduced to less than 25 and 15% with the application of single PAH/PSS and crosslinked PAH/PAA coatings, respectively, in comparison to at least a 45% loss observed with uncoated and PDDA/PSS-coated microspheres. Furthermore, it was found that enzyme within PAH/PSS- and crosslinked PAH/PAA-coated spheres retained more than 84 and 60% of initial activity, respectively, after three months, whereas uncoated and PDDA/PSS-coated microspheres retained less than 20%.

摘要

为了提高生物传感器长期使用的稳定性，研究了逐层自组装技术，该技术可作为一种潜在方法，为采用乳化技术制备的藻酸盐微球（<5微米）内封装的葡萄糖氧化酶提供扩散屏障。与未涂层和聚二烯丙基二甲基氯化铵/聚苯乙烯磺酸钠（PDDA/PSS）涂层微球至少45%的损失相比，分别应用单一的聚烯丙基胺盐酸盐/聚苯乙烯磺酸钠（PAH/PSS）和交联的PAH/聚丙烯酸（PAA）涂层后，封装酶的总损失分别降至25%和15%以下。此外，还发现，PAH/PSS涂层球和交联PAH/PAA涂层球内的酶在三个月后分别保留了超过84%和60%的初始活性，而未涂层和PDDA/PSS涂层微球保留的活性不到20%。