Yang Lung-Jieh, Ou Yu-Cheng
Department of Mechanical & Electro-Mechanical Engineering, Tamkang University, Tamsui, Taiwan R.O.C.
Lab Chip. 2005 Sep;5(9):979-84. doi: 10.1039/b505193b. Epub 2005 Aug 2.
This paper proposes a novel technique for fabricating micro patterns of glutaraldehyde (GA)-crosslinked gelatin. It provides another means to crosslink gelatin other than using photo-sensitizing agents, and the micro patterns of GA-crosslinked gelatin can still be made successfully by accessing conventional photolithography. A much less toxic and increased biocompatible approach to strengthening the gelatin microstructures can be developed according to this idea. This paper also describes a potential methodology for using GA-crosslinked gelatin patterns as single-cell culture bases. The best spatial resolution of the micro gelatin bases can reach 10 microm, and the selective growing density of human Mesenchymal stem cells on the gelatin patterns surpasses the density on the glass substrate by 2-3 orders of magnitude.
本文提出了一种用于制造戊二醛（GA）交联明胶微图案的新技术。它提供了一种不使用光敏剂交联明胶的方法，并且通过传统光刻技术仍能成功制作GA交联明胶的微图案。根据这一思路，可以开发出一种毒性更小、生物相容性更高的增强明胶微结构的方法。本文还描述了一种将GA交联明胶图案用作单细胞培养基底的潜在方法。微明胶基底的最佳空间分辨率可达10微米，人骨髓间充质干细胞在明胶图案上的选择性生长密度比在玻璃基板上的密度高出2 - 3个数量级。
