Tomić Simonida Lj, Nikodinović-Runić Jasmina, Vukomanović Marija, Babić Marija M, Vuković Jovana S
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11000 Belgrade, Serbia.
Polymers (Basel). 2021 Mar 18;13(6):932. doi: 10.3390/polym13060932.
Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material's composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.
水凝胶支架生物材料是再生工程中最具吸引力的聚合物生物材料之一，由于其与天然细胞外基质相似，可被设计成组织模拟支架以支持细胞生长。基于藻酸盐、明胶、甲基丙烯酸2-羟乙酯和无机试剂羟基磷灰石的新型多功能水凝胶支架通过改良冷冻凝胶化法制备。测试了其化学成分、形态、孔隙率、力学性能、对细胞活力的影响、体外降解、体外和体内生物相容性，以关联材料组成与相应性能。支架呈现出相互连通的多孔微观结构、令人满意的机械强度、良好的亲水性、降解性以及合适的体外和体内生物相容性。材料在细胞培养中与健康人成纤维细胞表现出良好的生物相容性，在斑马鱼实验中体内生物相容性也良好，表明新合成的水凝胶支架作为新一代具有可调性能的水凝胶支架生物材料，可用于多种生物医学应用和组织再生。
