Borsari Veronica, Giavaresi Gianluca, Fini Milena, Torricelli Paola, Salito Armando, Chiesa Roberto, Chiusoli Loris, Volpert Andreas, Rimondini Lia, Giardino Roberto
Department of Experimental Surgery, Research Institute Codivilla-Putti, Rizzoli Orthopaedic Institute, Bologna, Italy.
J Biomed Mater Res B Appl Biomater. 2005 Nov;75(2):359-68. doi: 10.1002/jbm.b.30313.
The aim of this study was to characterize and compare various titanium (Ti) and hydroxyapatite (HA) coatings on Ti6Al4V, in view of their application on noncemented orthopedic implants. Two innovative vacuum plasma sprayed (VPS) coatings, the first of ultrahigh rough and dense Ti (PG60, Ra=74 microm) and the second of ultrahigh rough and dense Ti coated with HA (HPG60, Ra=52 microm), have been developed, and the response of osteoblast-like cells (MG-63) seeded on these new coatings was evaluated in comparison to: a low roughness and sandblasted (Ti/SA, Ra=4 microm) Ti6Al4V surface; Ti medium (TI01, Ra=18 microm), and high (TI60, Ra=40 microm) roughness VPS coatings; and the relative Ti plus HA duplex coatings (HT01, Ra=12 microm and HT60, Ra=36 microm respectively), also obtained by VPS. PG60 coating presented no open porosity, making it dense and potentially intrinsically stronger. Cell adhesion and proliferation on PG60 was similar to those of the smoothest one (Ti/SA) and adhesion on ultrahigh roughness was lower than the medium- and high-roughness coatings, whereas cell proliferation on PG60 was lower than TI60. The HA coating determined significant increases in cell proliferation at medium and high roughness levels when compared to the relative Ti coating, but not compared to the ultrahigh one; all HA-coated surfaces showed a decrease in alkaline phosphatase activity and collagen I production. Surface morphology and the HA coating strongly affected cell behavior. However, ultrahigh values of roughness are not correctly seen by cells, and the presence of HA has no improving effects.
本研究的目的是鉴于其在非骨水泥型骨科植入物上的应用，对Ti6Al4V上的各种钛（Ti）和羟基磷灰石（HA）涂层进行表征和比较。已经开发了两种创新的真空等离子喷涂（VPS）涂层，第一种是超高粗糙度和致密的Ti（PG60，表面粗糙度算术平均值Ra = 74微米），第二种是涂有HA的超高粗糙度和致密的Ti（HPG60，Ra = 52微米），并将接种在这些新涂层上的成骨样细胞（MG-63）的反应与以下情况进行了比较：低粗糙度喷砂处理（Ti/SA，Ra = 4微米）的Ti6Al4V表面；中等粗糙度（TI01，Ra = 18微米）和高粗糙度（TI60，Ra = 40微米）的VPS涂层；以及同样通过VPS获得的相对的Ti加HA双相涂层（HT01，Ra = 12微米和HT60，Ra = 36微米）。PG60涂层没有开孔孔隙，使其致密且可能本质上更强。PG60上的细胞粘附和增殖与最光滑的涂层（Ti/SA）相似，超高粗糙度上的粘附低于中等粗糙度和高粗糙度涂层，而PG60上的细胞增殖低于TI60。与相对的Ti涂层相比，HA涂层在中等和高粗糙度水平下使细胞增殖显著增加，但与超高粗糙度涂层相比则不然；所有HA涂层表面的碱性磷酸酶活性和I型胶原蛋白生成均降低。表面形态和HA涂层强烈影响细胞行为。然而，细胞无法正确识别超高的粗糙度值，并且HA的存在没有改善作用。
