金黄色葡萄球菌中两种脯氨酸转运系统的鉴定及其在渗透调节中的可能作用。
Identification of two proline transport systems in Staphylococcus aureus and their possible roles in osmoregulation.
作者信息
Bae J H, Miller K J
机构信息
Department of Food Science, Pennsylvania State University, University Park 16802.
出版信息
Appl Environ Microbiol. 1992 Feb;58(2):471-5. doi: 10.1128/aem.58.2.471-475.1992.
The food-borne pathogen Staphylococcus aureus is distinguished from other food-borne pathogens by its ability to grow at water activity values below 0.90. Previous studies have indicated that proline accumulation mediated by transport represents a primary osmoregulatory strategy utilized by this bacterium (C. B. Anderson and L. D. Witter, Appl. Environ, Microbiol. 43:1501-1503, 1982; I. Koujima, H. Hayashi, K. Tomochika, A. Okabe, and Y. Kanemasa, Appl. Environ. Microbiol. 35:467-470, 1978; K. J. Miller, S. C. Zelt, and J.-H. Bae, Curr. Microbiol. 23:131-137, 1991). In this study, we demonstrate the presence of two proline transport systems within whole cells of S. aureus, a high-affinity transport system (Km, 7 microM) and a low-affinity transport system (Km, 420 microM). Our results indicate that the low-affinity proline transport system is osmotically activated and is the primary system responsible for the accumulation of proline by this pathogen during growth at low water activity.
食源性病原体金黄色葡萄球菌与其他食源性病原体的区别在于其能够在水分活度值低于0.90的条件下生长。先前的研究表明，由转运介导的脯氨酸积累是该细菌利用的一种主要渗透调节策略（C.B.安德森和L.D.维特，《应用与环境微生物学》43:1501 - 1503，1982年；I.小岛、H.林、K.友近、A.冈部和Y.金政，《应用与环境微生物学》35:467 - 470，1978年；K.J.米勒、S.C.泽尔特和J.-H.裴，《微生物学通报》23:131 - 137，1991年）。在本研究中，我们证明了金黄色葡萄球菌全细胞内存在两种脯氨酸转运系统，一种是高亲和力转运系统（Km，7微摩尔）和一种低亲和力转运系统（Km，420微摩尔）。我们的结果表明，低亲和力脯氨酸转运系统受到渗透激活，并且是该病原体在低水分活度下生长期间脯氨酸积累的主要负责系统。
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