Amaral André Corrêa, Fernandes Larissa, Galdino Alexsandro Sobreira, Felipe Maria Sueli Soares, Soares Célia Maria de Almeida, Pereira Maristela
Departamento de Biologia Celular, IB, Universidade de Brasília, Brasília, DF, Brazil.
Genet Mol Res. 2005 Jun 30;4(2):430-49.
The rise in antifungal resistance, observed as a result of the increasing numbers of immunocompromised patients, has made the discovery of new targets for drug therapy imperative. The description of the Paracoccidioides brasiliensis transcriptome has allowed us to find alternatives to refine current therapy against paracoccidioidomycosis. We used comparative analysis of expressed sequence tags to find promising drug targets that have been addressed in other pathogens. We divided the analysis into six different categories, based on the involvement of the targeted mechanisms in the cell: i) cell wall construction, ii) plasma membrane composition, iii) cellular machinery, iv) cellular metabolism, v) signaling pathways, and vi) other essential processes. Through this approach, it has been possible to infer strategies to develop alternative drugs against this pathogen.
由于免疫功能低下患者数量的增加，观察到抗真菌耐药性的上升，这使得发现药物治疗的新靶点势在必行。巴西副球孢子菌转录组的描述使我们能够找到改进当前治疗副球孢子菌病方法的替代方案。我们使用表达序列标签的比较分析来寻找在其他病原体中已被研究的有前景的药物靶点。基于靶向机制在细胞中的作用，我们将分析分为六个不同类别：i）细胞壁构建，ii）质膜组成，iii）细胞机制，iv）细胞代谢，v）信号通路，以及vi）其他基本过程。通过这种方法，已经有可能推断出开发针对这种病原体的替代药物的策略。
