McClenny N
CLS Internship Program, San Francisco State University, San Francisco, CA, USA.
Med Mycol. 2005 May;43 Suppl 1:S125-8. doi: 10.1080/13693780500052222.
Molecular and immunologic tests promise better, faster laboratory diagnosis of aspergillosis, but microscopy and culture remain commonly used and essential tools. Procedural changes, as well as adequate training of laboratory professionals, can enhance the value of these traditional tools. Using Blankophor or Calcofluor for microscopic examinations; improving recognition of morphologic characteristics of opportunistic fungi in stained smears of specimens; maximizing the growth rate and production of conidia by Aspergillus spp. in culture; and recognizing atypical variants of common aspergilli can improve the laboratory's contribution to rapid diagnosis. Surveys indicate that the number of laboratory professionals is declining as the demand for healthcare is rising. Effective recruitment, retention, and training of personnel must be concurrent with advances in technology.
分子和免疫检测有望实现对曲霉病更好、更快的实验室诊断，但显微镜检查和培养仍是常用且必不可少的工具。程序上的改变以及对实验室专业人员的充分培训，可以提高这些传统工具的价值。使用荧光增白剂或钙荧光白进行显微镜检查；提高在标本染色涂片中对机会性真菌形态特征的识别能力；在培养中最大化曲霉属菌种的生长速度和分生孢子产量；以及识别常见曲霉的非典型变体，都可以提高实验室对快速诊断的贡献。调查表明，随着医疗保健需求的上升，实验室专业人员的数量正在减少。有效的人员招聘、留用和培训必须与技术进步同步进行。
