Adams J, Puskas-Rozsa S, Simlar J, Wilke C M
Department of Biology, University of Michigan, Ann Arbor 48109.
Curr Genet. 1992 Jul;22(1):13-9. doi: 10.1007/BF00351736.
Thirteen independent populations of Saccharomyces cerevisiae (nine haploid and four diploid) were maintained in continuous culture for up to approximately 1000 generations, with growth limited by the concentration of organic phosphates in medium buffered at pH 6. Analysis of clones isolated from these populations showed that a number (17) of large-scale chromosomal-length variants and rearrangements were present in the populations at their termination. Nine of the 16 yeast chromosomes were involved in such changes. Few of the changes could be explained by copy-number increases in the structural loci for acid phosphatase. Several considerations concerning the nature and frequency of the chromosome-length variants observed lead us to conclude that they are selectively advantageous.
对13个独立的酿酒酵母群体（9个单倍体和4个二倍体）进行连续培养，持续约1000代，其生长受pH 6缓冲培养基中有机磷酸盐浓度的限制。对从这些群体中分离出的克隆进行分析表明，在培养结束时，群体中存在一些（17个）大规模的染色体长度变异和重排。16条酵母染色体中有9条参与了此类变化。很少有变化可以用酸性磷酸酶结构基因座的拷贝数增加来解释。关于所观察到的染色体长度变异的性质和频率的几点考虑使我们得出结论，它们具有选择优势。
