Leon P, O'Brien-Vedder C, Walbot V
Department of Biological Sciences, Stanford University, CA 94305-5020.
Curr Genet. 1992 Jul;22(1):61-7. doi: 10.1007/BF00351743.
ORF1 of the ubiquitous 2.3 kb linear plasmid of maize mitochondria encodes a 39 kDa protein detected with polyclonal antibodies raised to a beta-galactosidase:ORF1 fusion protein. Almost half of this protein is similar to a domain of the 130 kDa protein encoded by the S2 episome of mitochondria from cytoplasmic male-sterile lines. Antisera raised to the ORF1 2.3 kb plasmid product cross-reacts with the ORF1, 130 kDa protein from the S2 episome. Despite the shared domain, the proteins are differentially localized: the 130 kDa protein is membrane-associated while the ORF1 protein is found in the matrix. We discuss possible functions of the ORF1 protein.
玉米线粒体中普遍存在的2.3 kb线性质粒的ORF1编码一种39 kDa的蛋白质，该蛋白质可通过针对β-半乳糖苷酶：ORF1融合蛋白产生的多克隆抗体检测到。这种蛋白质几乎一半与细胞质雄性不育系线粒体的S2附加体编码的130 kDa蛋白质的一个结构域相似。针对ORF1 2.3 kb质粒产物产生的抗血清与S2附加体的ORF1、130 kDa蛋白质发生交叉反应。尽管存在共享结构域，但这些蛋白质的定位不同：130 kDa蛋白质与膜相关，而ORF1蛋白质存在于基质中。我们讨论了ORF1蛋白质的可能功能。
