Allander Tobias, Tammi Martti T, Eriksson Margareta, Bjerkner Annelie, Tiveljung-Lindell Annika, Andersson Björn
Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12891-6. doi: 10.1073/pnas.0504666102. Epub 2005 Aug 23.
The identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR amplification, large-scale sequencing, and bioinformatics. The technology was applied to pooled human respiratory tract samples. The first experiments detected seven human virus species without the use of any specific reagent. Among the detected viruses were one coronavirus and one parvovirus, both of which were at that time uncharacterized. The parvovirus, provisionally named human bocavirus, was in a retrospective clinical study detected in 17 additional patients and associated with lower respiratory tract infections in children. The molecular virus screening procedure provides a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples. We suggest that a systematic exploration of the viruses that infect humans, "the human virome," can be initiated.
新病毒种类的鉴定是传染病研究的关键问题，但在技术上非常困难。我们基于宿主DNA去除、随机PCR扩增、大规模测序和生物信息学，开发了一种用于临床样本大规模分子病毒筛查的系统。该技术应用于合并的人类呼吸道样本。首次实验在未使用任何特异性试剂的情况下检测到了7种人类病毒。在检测到的病毒中有1种冠状病毒和1种细小病毒，当时这两种病毒均未被鉴定。这种细小病毒被暂命名为人类博卡病毒，在一项回顾性临床研究中，又在另外17名患者中检测到，且与儿童下呼吸道感染有关。分子病毒筛查程序为检测单个或合并样本中未知病毒种类的问题提供了一种通用的非培养方法。我们建议可以启动对感染人类的病毒“人类病毒组”的系统探索。
