De S, Sharma R, Trigwell S, Laska B, Ali N, Mazumder M K, Mehta J L
Department of Applied Science, University of Arkansas at Little Rock, 2801 South University, Little Rock, AR 72204, USA.
J Biomater Sci Polym Ed. 2005;16(8):973-89. doi: 10.1163/1568562054414612.
The advantage of helium plasma treatment in enhancing endothelial cell growth and adhesion on polyurethane film coated on glass substrate is demonstrated with experimental data. Human coronary artery endothelial cell (HCAE) growth and attachment was studied on (1) bare glass substrate, used as control, (2) coated glass, with and without helium plasma treatment and (3) collagen-treated polyurethane-coated glass substrates. The untreated polyurethane film surface was rough (RMS = 690 nm) and highly hydrophobic (contact angle theta = 90 degrees). Cell growth on the untreated polyurethane surface was poor (cell concentration approximately 3750/cm2) compared to glass surface (cell concentration approximately 17 665/cm2). The atmospheric helium plasma treatment of the polyurethane film resulted in oxidation of the surface, a slight increase in roughness (RMS = 735 nm) and a significant drop in hydrophobicity (contact angle theta = 79 degrees). The critical surface tension (gamma c) of polyurethane film was also increased by 2 dynes/cm due to helium plasma treatment. These changes resulted in enhanced HCAE cell growth in polyurethane film (cell concentration approximately 16 230/cm2) compared to the untreated polyurethane film. The cell growth was also comparable to cell growth on a glass surface (17 665/cm2) and the collagen-treated polyurethane film surfaces (cell concentration approximately 21 645/cm2), respectively. Moreover, the strength of cell attachment on a plasma-treated surface (cell retention R = 89%) under laminar flow was significantly higher than that on a glass surface (R = 71%). While the collagen-treated polyurethane surface had the highest number of HCAE cells, the cell adhesion was found to be poor (R = 42%) compared to that of a plasma-treated surface. Thus, the overall performance of the plasma-treated polyurethane film surface on endothelial cell growth was better than other substrates studied here.
实验数据证明了氦等离子体处理在增强内皮细胞在涂覆于玻璃基板上的聚氨酯薄膜上的生长和黏附方面的优势。研究了人冠状动脉内皮细胞（HCAE）在以下几种表面上的生长和附着情况：（1）用作对照的裸玻璃基板；（2）涂覆玻璃，有无氦等离子体处理；（3）胶原处理的聚氨酯涂覆玻璃基板。未处理的聚氨酯薄膜表面粗糙（均方根粗糙度RMS = 690 nm）且高度疏水（接触角θ = 90度）。与玻璃表面（细胞浓度约为17665/cm²）相比，未处理的聚氨酯表面上的细胞生长较差（细胞浓度约为3750/cm²）。对聚氨酯薄膜进行大气氦等离子体处理导致表面氧化，粗糙度略有增加（RMS = 735 nm），疏水性显著下降（接触角θ = 79度）。由于氦等离子体处理，聚氨酯薄膜的临界表面张力（γc）也增加了2达因/厘米。这些变化导致与未处理的聚氨酯薄膜相比，HCAE细胞在聚氨酯薄膜中的生长增强（细胞浓度约为16230/cm²）。细胞生长也分别与玻璃表面（17665/cm²）和胶原处理的聚氨酯薄膜表面（细胞浓度约为21645/cm²）上的细胞生长相当。此外，在层流条件下，等离子体处理表面上的细胞附着强度（细胞保留率R = 89%）显著高于玻璃表面（R = 71%）。虽然胶原处理的聚氨酯表面上的HCAE细胞数量最多，但与等离子体处理表面相比，发现细胞黏附较差（R = 42%）。因此，等离子体处理的聚氨酯薄膜表面在内皮细胞生长方面的整体性能优于此处研究的其他基板。
