VITEK 2比色卡用于革兰氏阳性菌和革兰氏阴性菌鉴定的性能
Performances of VITEK 2 colorimetric cards for identification of gram-positive and gram-negative bacteria.
作者信息
Wallet Frédéric, Loïez Caroline, Renaux Emilie, Lemaitre Nadine, Courcol René J
机构信息
Laboratoire de Bactériologie-Hygiène, Hôpital A. Calmette, Lille, France.
出版信息
J Clin Microbiol. 2005 Sep;43(9):4402-6. doi: 10.1128/JCM.43.9.4402-4406.2005.
Abstract
The purpose of this study was to evaluate the new VITEK 2 identification cards that use colorimetric reading to identify gram-positive and gram-negative bacteria (GP and GN cards, respectively) in comparison to fluorimetric cards (ID-GPC and ID-GNB, respectively). A total of 580 clinical isolates and stock collection strains belonging to 116 taxa were included in the study. Of the 249 gram-positive strains tested with both the ID-GPC and GP cards, 218 (87.5%) and 235 (94.4%) strains were correctly identified (to the genus and species level), respectively. Of the 331 gram-negative strains tested with the ID-GNB and GN cards, 295 (89.1%) and 321 (97%) strains were correctly identified, respectively. Another focus of the study was to apply the percentages of correct identifications obtained in this study to the list of bacteria isolated in our laboratory (32,739 isolates) in the year 2004. We obtained 97.9% correct identifications with the colorimetric cards and 93.9% with fluorescent cards.
摘要
本研究的目的是评估新型VITEK 2鉴定卡，该鉴定卡利用比色读数分别鉴定革兰氏阳性菌和革兰氏阴性菌（分别为GP卡和GN卡），并与荧光卡（分别为ID-GPC和ID-GNB）进行比较。本研究共纳入了属于116个分类单元的580株临床分离株和保藏菌株。在用ID-GPC卡和GP卡检测的249株革兰氏阳性菌株中，分别有218株（87.5%）和235株（94.4%）被正确鉴定（到属和种水平）。在用ID-GNB卡和GN卡检测的331株革兰氏阴性菌株中，分别有295株（89.1%）和321株（97%）被正确鉴定。本研究的另一个重点是将本研究中获得的正确鉴定百分比应用于我们实验室2004年分离的细菌列表（32,739株分离株）。我们用比色卡获得了97.9%的正确鉴定率，用荧光卡获得了93.9%的正确鉴定率。
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