Rae J L, Levis R A
Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905.
Pflugers Arch. 1992 Apr;420(5-6):618-20. doi: 10.1007/BF00374642.
We present a method whereby, with integrating electronics, quartz patch electrodes and a novel use of silicone oil, background noise levels as low as .083 pA RMS in a 5 kHz bandwidth (4-pole Butterworth filter) have been achieved in single channel patch clamp recordings. These approaches result in much higher signal to noise ratios for single channel recording than have previously been reported and should allow many investigators to significantly reduce noise at a constant bandwidth or to increase their recording bandwidths by several kHz.
我们提出了一种方法，通过集成电子器件、石英膜片电极以及硅油的新用途，在单通道膜片钳记录中，于5 kHz带宽（4阶巴特沃斯滤波器）下实现了低至0.083 pA RMS的背景噪声水平。这些方法使得单通道记录的信噪比远高于先前报道的水平，应该能让许多研究人员在恒定带宽下显著降低噪声，或者将记录带宽提高几个kHz。
