通过磷光寿命成像测量小鼠眼睛中的氧分布和血管损伤。
Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging.
作者信息
Wilson David F, Vinogradov Sergei A, Grosul Pavel, Vaccarezza M Noel, Kuroki Akiko, Bennett Jean
机构信息
Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
出版信息
Appl Opt. 2005 Sep 1;44(25):5239-48. doi: 10.1364/ao.44.005239.
Abstract
Maps of the oxygen distribution in the retina of the mouse eye were obtained by phosphorescence-lifetime imaging. Phosphor dissolved in the blood was excited by modulated light and phosphorescence imaged through microscope optics with an intensified-CCD camera. Phosphorescence lifetimes and oxygen pressures were calculated for each pixel of the images. The resolution was sufficient to permit the detection of anomalies that result in reduced oxygen pressures in individual retinal capillaries. High-resolution maps of oxygen distribution in the retina can provide greater understanding of the role of oxygen and vascular function in diseases of the eye.
摘要
通过磷光寿命成像获得了小鼠眼睛视网膜中氧气分布的图谱。溶解在血液中的磷光体由调制光激发，并通过显微镜光学系统用增强型电荷耦合器件相机对磷光进行成像。计算图像中每个像素的磷光寿命和氧气压力。该分辨率足以检测导致单个视网膜毛细血管中氧气压力降低的异常情况。视网膜中氧气分布的高分辨率图谱可以更深入地了解氧气和血管功能在眼部疾病中的作用。
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