Versteegh F G A, Weverling G J, Peeters M F, Wilbrink B, Veenstra-van Schie M T M, van Leeuwen-Gerritsen J M, Mooi-Kokenberg E A N M, Schellekens J F P, Roord J J
Groene Hart Ziekenhuis, Department of Pediatrics, Gouda, the Netherlands.
Clin Microbiol Infect. 2005 Oct;11(10):801-7. doi: 10.1111/j.1469-0691.2005.01234.x.
A 2-year prospective study was performed of children with prolonged coughing to investigate the frequency of different respiratory pathogens, the rate of mixed infections, and possible differences in severity of disease between single and mixed infections. Sera from 135 children (136 episodes of prolonged coughing lasting 1-6 weeks) were tested for antibodies to different viruses and bacteria. Swabs were taken for culture and PCR to detect different viral and bacterial pathogens. One or more pathogens were found in 91 (67%) patients. One infectious agent was found in 49 (36%) patients, two agents in 35 (26%) patients, and more than two agents in seven (5%) patients. The most frequent pathogens encountered were rhinovirus (n = 43; 32%), Bordetella pertussis (n = 23; 17%) and respiratory syncytial virus (n = 15; 11%). The most frequent mixed infection was B. pertussis and rhinovirus (n = 14; 10%). No significant differences in clinical symptoms were observed between patients with or without pathogens; however, patients with mixed infections were significantly older. There was a strong seasonal influence on the number of infections, but not on the number of mixed infections. In children with prolonged coughing, there was a high frequency of mixed infections regardless of the season. However, mixed infection was not associated with increased disease severity. No clinical symptoms were found that allowed discrimination between specific pathogens.
对患有持续性咳嗽的儿童进行了一项为期2年的前瞻性研究，以调查不同呼吸道病原体的感染频率、混合感染率以及单一感染和混合感染之间疾病严重程度的可能差异。对135名儿童（136例持续1 - 6周的持续性咳嗽发作）的血清进行了不同病毒和细菌抗体检测。采集拭子进行培养和PCR检测，以发现不同的病毒和细菌病原体。91名（67%）患者中发现了一种或多种病原体。49名（36%）患者发现一种感染病原体，35名（26%）患者发现两种病原体，7名（5%）患者发现两种以上病原体。最常见的病原体是鼻病毒（n = 43；32%）、百日咳博德特氏菌（n = 23；17%）和呼吸道合胞病毒（n = 15；11%）。最常见的混合感染是百日咳博德特氏菌和鼻病毒（n = 14；10%）。有无病原体的患者之间未观察到临床症状有显著差异；然而，混合感染的患者年龄显著更大。感染数量受季节影响较大，但混合感染数量不受季节影响。在患有持续性咳嗽的儿童中，无论季节如何，混合感染的频率都很高。然而，混合感染与疾病严重程度增加无关。未发现可区分特定病原体的临床症状。
